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Cold-atmospheric plasma induces tumor cell death in preclinical in vivo and in vitro models of human cholangiocarcinoma

التفاصيل البيبلوغرافية
العنوان: Cold-atmospheric plasma induces tumor cell death in preclinical in vivo and in vitro models of human cholangiocarcinoma
المؤلفون: Jérémy Augustin, Fatiha Merabtene, Javier Vaquero, Florian Judée, Ander Arbelaiz, Chantal Housset, Laura Fouassier, Ester Gonzalez-Sanchez, Marie Vallette, Henri Decauchy, Thierry Dufour, Olivier Scatton, Lynda Aoudjehane
المساهمون: Centre de Recherche Saint-Antoine (CR Saint-Antoine), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Laboratoire de Physique des Plasmas (LPP), Observatoire de Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École polytechnique (X)-Sorbonne Université (SU)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Institut d'Investigació Biomèdica de Bellvitge [Barcelone] (IDIBELL), Unité de Recherche sur les Maladies Cardiovasculaires, du Métabolisme et de la Nutrition = Institute of cardiometabolism and nutrition (ICAN), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Service d'Anatomie et cytologie pathologiques [CHU Pitié-Salpêtrière] (ACP), Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Sorbonne Université (SU), Institut National de la Santé et de la Recherche Médicale (INSERM)
المصدر: Dipòsit Digital de la UB
Universidad de Barcelona
Cancers, Vol 12, Iss 1280, p 1280 (2020)
Cancers
Volume 12
Issue 5
Cancers, MDPI, 2020, 12 (5), pp.1280. ⟨10.3390/cancers12051280⟩
بيانات النشر: arXiv, 2020.
سنة النشر: 2020
مصطلحات موضوعية: 0301 basic medicine, Cancer Research, Programmed cell death, Cell cycle checkpoint, Macròfags, tumor cells, Macrophage polarization, Context (language use), [SDV.CAN]Life Sciences [q-bio]/Cancer, Therapeutics, cold plasma, lcsh:RC254-282, Article, 03 medical and health sciences, 0302 clinical medicine, In vivo, Càncer, Tissues and Organs (q-bio.TO), innovative therapy, Cancer, Tumor microenvironment, Chemistry, plasma jet, Macrophages, [SPI.PLASMA]Engineering Sciences [physics]/Plasmas, Quantitative Biology - Tissues and Organs, Cell cycle, plasma selectivity, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, Terapèutica, 3. Good health, macrophages, 030104 developmental biology, Oncology, Apoptosis, 030220 oncology & carcinogenesis, FOS: Biological sciences, Cancer research, [SDV.IB]Life Sciences [q-bio]/Bioengineering, cholangiocarcinoma
الوصف: Through the last decade, cold atmospheric plasma (CAP) has emerged as an innovative therapeutic option for cancer treatment. Recently, we have set up a potentially safe atmospheric pressure plasma jet device that displays antitumoral properties in a preclinical model of cholangiocarcinoma (CCA), a rare and very aggressive cancer emerging from the biliary tree with few efficient treatments. In the present study, we aimed at deciphering the molecular mechanisms underlying the antitumor effects of CAP towards CCA in both an in vivo and in vitro context. In vivo, using subcutaneous xenografts into immunocompromised mice, CAP treatment of CCA induced DNA lesions and tumor cell apoptosis, as evaluated by 8-oxoguanine and cleaved caspase-3 immunohistochemistry, respectively. The analysis of the tumor microenvironment showed changes in markers related to macrophage polarization. In vitro, the incubation of CCA cells with CAP-treated culture media (i.e., plasma-activated media, PAM) led to a dose response decrease in cell survival. At molecular level, CAP treatment induced double-strand DNA breaks, followed by an increased phosphorylation and activation of the cell cycle master regulators CHK1 and p53, leading to cell cycle arrest and cell death by apoptosis. In conclusion, CAP is a novel therapeutic option to consider for CCA in the future.
وصف الملف: application/pdf
تدمد: 2072-6694
DOI: 10.48550/arxiv.2007.15093
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5d712709a1d26868507cd4c8fcdbf7f3
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....5d712709a1d26868507cd4c8fcdbf7f3
قاعدة البيانات: OpenAIRE
الوصف
تدمد:20726694
DOI:10.48550/arxiv.2007.15093