Biochemical and proteomic analyses of the physiological response induced by individual housing in gilts provide new potential stress markers
| العنوان: | Biochemical and proteomic analyses of the physiological response induced by individual housing in gilts provide new potential stress markers |
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| المؤلفون: | Anna Bassols, Laura Arroyo, Yolanda Saco, Emøke Bendixen, Raquel Peña, Raquel Pato, Lorenzo Fraile, Anna Marco-Ramell |
| المصدر: | Marco-Ramell, A, Arroyo, L, Peña, R, Pato, R, Saco, Y, Fraile, L, Bendixen, E & Bassols, A 2016, ' Biochemical and proteomic analyses of the physiological response induced by individual housing in gilts provide new potential stress markers ', BMC Veterinary Research, vol. 12, 265 . https://doi.org/10.1186/s12917-016-0887-1 Dipòsit Digital de la UB Universidad de Barcelona BMC Veterinary Research Recercat. Dipósit de la Recerca de Catalunya instname Repositorio Abierto de la UdL Universitad de Lleida Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona |
| بيانات النشر: | Springer Science and Business Media LLC, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Male, Estrès, Proteomics, 0301 basic medicine, medicine.medical_specialty, Proteome, GPX3, Swine, Immunoteràpia, Protein metabolism, Acute phase proteins, Peroxiredoxin 2, Immunotheraphy, Biology, Animal Welfare, Stress, medicine.disease_cause, 03 medical and health sciences, chemistry.chemical_compound, Stress, Physiological, Internal medicine, medicine, Animals, Electrophoresis, Gel, Two-Dimensional, Innate immunity, Pig, Innate immune system, Staining and Labeling, General Veterinary, Biochemical markers, Acute-phase protein, Biomarker, General Medicine, Housing, Animal, veterinary(all), Lipids, Lactotransferrin, Protein catabolism, 030104 developmental biology, Endocrinology, chemistry, Oxidative stress, Marcadors bioquímics, Immunology, Individual confinement, Biomarkers, Research Article |
| الوصف: | Background The objective assessment of animal stress and welfare requires proper laboratory biomarkers. In this work, we have analyzed the changes in serum composition in gilts after switching their housing, from pen to individual stalls, which is generally accepted to cause animal discomfort. Results Blood and saliva samples were collected a day before and up to four days after changing the housing system. Biochemical analyses showed adaptive changes in lipid and protein metabolism after the housing switch, whereas cortisol and muscular markers showed a large variability between animals. 2D-DIGE and iTRAQ proteomic approaches revealed variations in serum protein composition after changing housing and diet of gilts. Both techniques showed alterations in two main homeostatic mechanisms: the innate immune and redox systems. The acute phase proteins haptoglobin, apolipoprotein A-I and α1-antichymotrypsin 3, and the antioxidant enzyme peroxiredoxin 2 were found differentially expressed by 2D-DIGE. Other proteins related to the innate immune system, including lactotransferrin, protegrin 3 and galectin 1 were also identified by iTRAQ, as well as oxidative stress enzymes such as peroxiredoxin 2 and glutathione peroxidase 3. Proteomics also revealed the decrease of apolipoproteins, and the presence of intracellular proteins in serum, which may indicate physical injury to tissues. Conclusions Housing of gilts in individual stalls and diet change increase lipid and protein catabolism, oxidative stress, activate the innate immune system and cause a certain degree of tissue damage. We propose that valuable assays for stress assessment in gilts may be based on a score composed by a combination of salivary cortisol, lipid metabolites, innate immunity and oxidative stress markers and intracellular proteins. Electronic supplementary material The online version of this article (doi:10.1186/s12917-016-0887-1) contains supplementary material, which is available to authorized users. |
| وصف الملف: | application/pdf |
| تدمد: | 1746-6148 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5d8fd2cd6a843ffc679f2179998e16b4 https://doi.org/10.1186/s12917-016-0887-1 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....5d8fd2cd6a843ffc679f2179998e16b4 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 17466148 |
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