A comparative proteomic analysis for adventitious root formation in lotus root (Nelumbo nuciferaGaertn)
| العنوان: | A comparative proteomic analysis for adventitious root formation in lotus root (Nelumbo nuciferaGaertn) |
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| المؤلفون: | Li Shuyan, Yang Mengli, Jiang Runzhi, Li Liangjun, Cheng Libao |
| المصدر: | Zeitschrift für Naturforschung C. 72:181-196 |
| بيانات النشر: | Walter de Gruyter GmbH, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Proteomics, 0106 biological sciences, 0301 basic medicine, Lotus, Nelumbo nucifera, Nelumbo, Mass spectrometry, 01 natural sciences, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, Gene Expression Regulation, Plant, Tandem Mass Spectrometry, Botany, Gene, Plant Proteins, Root formation, biology, Reverse Transcriptase Polymerase Chain Reaction, Chemistry, Lotus root, biology.organism_classification, Rhizome, 030104 developmental biology, Biochemistry, Seedling, Chromatography, Liquid, 010606 plant biology & botany |
| الوصف: | Adventitious roots (ARs) directly affect lotus seedling growth and product quality because principal root is not well developed. However, the details of AR formation at the molecular level have not been determined in lotus. Therefore, three stages were chosen to identify the change of proteins abundant during rhizome formation, using isobaric tags for relative and absolute quantization coupled with liquid chromatography–tandem mass spectrometry to gain insight into the molecular mechanisms involved in AR formation. We totally obtained 323,375 spectra during AR formation. After filtering to eliminate low-scoring spectra, 66,943 spectra, including 53,106 unique spectra, were identified. These unique spectra matched 28,905 peptides, including 24,992 unique peptides, which were assembled into 6686 proteins. In the C0/C1 and C1/C2 stages, 66 and 32 proteins showed enhanced abundance, and 173 and 73 proteins showed decreased abundance, respectively. Seventeen important AR formation-related proteins from the three stages were identified, and the expressions of nine genes from the above-identified proteins were assessed by qRT-PCR. This article provides a comprehensive understanding of the changes in metabolism during AR formation, and is helpful to accelerate the progress of breeding in fulture in lotus root. |
| تدمد: | 1865-7125 0939-5075 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5e514c21c9c44e1bf599e0de87343d7c https://doi.org/10.1515/znc-2016-0170 |
| رقم الأكسشن: | edsair.doi.dedup.....5e514c21c9c44e1bf599e0de87343d7c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18657125 09395075 |
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