Design and visualization of second-generation cyanoisoindole-based fluorescent strigolactone analogs
| العنوان: | Design and visualization of second-generation cyanoisoindole-based fluorescent strigolactone analogs |
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| المؤلفون: | Sofie Goormachtig, Anna M. Kaczmarek, Lukas Braem, Sylwia Magdalena Struk, Rik Van Deun, François-Didier Boyer, Melissa Van Overtveldt, Thomas S. A. Heugebaert, Christian V. Stevens, Kris Gevaert |
| المساهمون: | Université d’Opole, Universiteit Gent = Ghent University [Belgium] (UGENT), Dept Med Prot Res, Flanders Institute for Biotechnology, Center for Plant Systems Biology (PSB Center), Vlaams Instituut voor Biotechnologie [Ghent, Belgique] (VIB), Research Foundation - Flanders (FWO Vlaanderen) |
| المصدر: | Plant Journal Plant Journal, Wiley, 2019, 98 (1), pp.165-180. ⟨10.1111/tpj.14197⟩ |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Arabidopsis, Strigolactone, Repressor, parasitic seed germination, Germination, Plant Science, Protein degradation, fluorescence labeling, 01 natural sciences, smxl6 degradation, 03 medical and health sciences, Lactones, Plant Growth Regulators, In vivo, Genetics, Arabidopsis thaliana, Moiety, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, strigolactone, Fluorescent Dyes, biology, Arabidopsis Proteins, hypocotyl elongation, arabidopsis thaliana, Cell Biology, phelipanche ramosa, biology.organism_classification, isoindole, Fluorescence, Terpenoid, Hypocotyl, 030104 developmental biology, Biochemistry, pyrroloindole, Proteolysis, Seeds, 010606 plant biology & botany, Signal Transduction |
| الوصف: | International audience; Strigolactones (SLs) are a family of terpenoid allelochemicals that were recognized as plant hormones only a decade ago. They influence a myriad of both above- and below-ground developmental processes, and are an important survival strategy for plants in nutrient-deprived soils. A rapidly emerging approach to gain knowledge on hormone signaling is the use of traceable analogs. A unique class of labeled SL analogs was constructed, in which the original tricyclic lactone moiety of natural SLs is replaced by a fluorescent cyanoisoindole ring system. Biological evaluation as parasitic seed germination stimulant and hypocotyl elongation repressor proved the potency of the cyanoisoindole strigolactone analogs (CISAs) to be comparable to the commonly accepted standard GR24. Additionally, via a SMXL6 protein degradation assay, we provided molecular evidence that the compounds elicit SL-like responses through the natural signaling cascade. All CISAs were shown to exhibit fluorescent properties, and the high quantum yield and Stokes shift of the pyrroloindole derivative CISA-7 also enabled in vivo visualization in plants. In contrast to the previously reported fluorescent analogs, CISA-7 displays a large similarity in shape and structure with natural SLs, which renders the analog a promising tracer to investigate the spatiotemporal distribution of SLs in plants and fungi. |
| تدمد: | 1365-313X 0960-7412 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5e56f44af5cc3d3fb81810aca0732d20 https://pubmed.ncbi.nlm.nih.gov/30552776 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....5e56f44af5cc3d3fb81810aca0732d20 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1365313X 09607412 |
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