Optical mapping discerns genome wide DNA methylation profiles
العنوان: | Optical mapping discerns genome wide DNA methylation profiles |
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المؤلفون: | James A. Thomson, Rod Runnheim, David C. Schwartz, Konstantinos Potamousis, Dan Forrest, Shiguo Zhou, Gene E. Ananiev, Christopher Churas, Steve Goldstein, Veit Bergendahl |
المصدر: | BMC Molecular Biology BMC Molecular Biology, Vol 9, Iss 1, p 68 (2008) |
بيانات النشر: | Springer Science and Business Media LLC, 2008. |
سنة النشر: | 2008 |
مصطلحات موضوعية: | lcsh:QH426-470, Restriction Mapping, Genomics, Biology, Genome, Epigenesis, Genetic, 03 medical and health sciences, 0302 clinical medicine, Escherichia coli, Humans, Fluorometry, Epigenetics, lcsh:QH573-671, Molecular Biology, RNA-Directed DNA Methylation, Embryonic Stem Cells, Fluorescent Dyes, 030304 developmental biology, Epigenomics, Genetics, 0303 health sciences, lcsh:Cytology, Gene Expression Profiling, Methylation, DNA Methylation, lcsh:Genetics, 030220 oncology & carcinogenesis, DNA methylation, Illumina Methylation Assay, CpG Islands, Research Article |
الوصف: | Background Methylation of CpG dinucleotides is a fundamental mechanism of epigenetic regulation in eukaryotic genomes. Development of methods for rapid genome wide methylation profiling will greatly facilitate both hypothesis and discovery driven research in the field of epigenetics. In this regard, a single molecule approach to methylation profiling offers several unique advantages that include elimination of chemical DNA modification steps and PCR amplification. Results A single molecule approach is presented for the discernment of methylation profiles, based on optical mapping. We report results from a series of pilot studies demonstrating the capabilities of optical mapping as a platform for methylation profiling of whole genomes. Optical mapping was used to discern the methylation profile from both an engineered and wild type Escherichia coli. Furthermore, the methylation status of selected loci within the genome of human embryonic stem cells was profiled using optical mapping. Conclusion The optical mapping platform effectively detects DNA methylation patterns. Due to single molecule detection, optical mapping offers significant advantages over other technologies. This advantage stems from obviation of DNA modification steps, such as bisulfite treatment, and the ability of the platform to assay repeat dense regions within mammalian genomes inaccessible to techniques using array-hybridization technologies. |
تدمد: | 1471-2199 |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5e978df7ac7fd55a4ab6979e7657a605 https://doi.org/10.1186/1471-2199-9-68 |
حقوق: | OPEN |
رقم الأكسشن: | edsair.doi.dedup.....5e978df7ac7fd55a4ab6979e7657a605 |
قاعدة البيانات: | OpenAIRE |
تدمد: | 14712199 |
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