Chromatographic Techniques for the Separation of a Thrombocytopoiesis-Stimulating Factor from Aplastic Rats
| العنوان: | Chromatographic Techniques for the Separation of a Thrombocytopoiesis-Stimulating Factor from Aplastic Rats |
|---|---|
| المؤلفون: | E Sumereau-Dassin, J Bourebia |
| المصدر: | Pathophysiology of Haemostasis and Thrombosis. 15:182-188 |
| بيانات النشر: | S. Karger AG, 1985. |
| سنة النشر: | 1985 |
| مصطلحات موضوعية: | medicine.medical_specialty, Ion chromatography, Fractionation, Chromatography, DEAE-Cellulose, chemistry.chemical_compound, Physiology (medical), Internal medicine, medicine, Animals, Hydroxyurea, Platelet, Erythropoietin, Thrombopoietin, Glycoproteins, Chromatography, Anemia, Aplastic, Rats, Inbred Strains, Radioimmunoassay, Hematology, Phosphate, Rats, Molecular Weight, Endocrinology, chemistry, Sephadex, Chromatography, Gel, medicine.drug |
| الوصف: | Thrombocytopoietin seems to be only partially responsible for the regulation of platelet production. We determined precisely the differences between a second thrombocytopoiesis-stimulating factor (TSF2), and thrombocytopoietin (TSF1) and erythropoietin (Epo). Fractionation was carried out, first on a DEAE-cellulose phosphate column and then on a Sephadex G-75 column. Thrombocytopoietic activity in the various fractions was assessed using 75Se-methionine platelet incorporation into normal recipients. Epo concentrations were determined using a radioimmunoassay. We showed that the apparent molecular weight of TSF2 is 14,000 daltons. It differs from TSF1 (48,000 daltons) and from Epo (39,000 daltons). For doses of 8–12 mU Epo/rat, found in whole serum injected, no effect on thrombocytopoiesis was found. On the contrary, a significant effect (p < 0.01) was found when the same quantities of Epo present in the Sephadex G-75 F4’ fraction were injected (2–10 mU/rat). TSF2 can be separated from TSF1 and Epo, using biochemical techniques. |
| تدمد: | 1424-8840 1424-8832 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5eacd4978a85811479b5d3a4a42b7215 https://doi.org/10.1159/000215142 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....5eacd4978a85811479b5d3a4a42b7215 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14248840 14248832 |
|---|