Telethonin variants found in Brugada syndrome, J‐wave pattern ECG, and ARVC reduce peak Na v 1.5 currents in HEK‐293 cells
| العنوان: | Telethonin variants found in Brugada syndrome, J‐wave pattern ECG, and ARVC reduce peak Na v 1.5 currents in HEK‐293 cells |
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| المؤلفون: | Minoru Horie, Masaru Yamakawa, Takeshi Ueyama, Peng Sheng Chen, Takeru Makiyama, Isik Turker, Matteo Vatta, Tomohiko Ai, Akihiko Shimizu |
| المصدر: | Pacing and Clinical Electrophysiology. 43:838-846 |
| بيانات النشر: | Wiley, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | medicine.medical_specialty, 030204 cardiovascular system & hematology, Telethonin, sudden cardiac death, 03 medical and health sciences, 0302 clinical medicine, Internal medicine, ARVC, Medicine, 030212 general & internal medicine, telethonin, J wave, Brugada syndrome, business.industry, Brugada, Sodium channel, HEK 293 cells, General Medicine, Transfection, medicine.disease, Phenotype, Electrophysiology, Endocrinology, J-wave syndromes, Cardiology and Cardiovascular Medicine, business, arrhythmias |
| الوصف: | Background:Telethonin (TCAP) is a Z-disk protein that maintains cytoskeletal integrity and various signaling pathways in cardiomyocytes. TCAP is shown to modulate α-subunit of the human cardiac sodium channel (hNav 1.5) by direct interactions. Several TCAP variants are found in cardiomyopathies. We sought to investigate whether TCAP variants are associated with arrhythmia syndromes. Methods:Mutational analyses for TCAP were performed in 303 Japanese patients with Brugada syndrome, arrhythmogenic right ventricular cardiomyopathy, and J-wave pattern ECG. Using patch-clamp techniques, electrophysiological characteristics of hNav 1.5 were studied in HEK-293 cells stably expressing hNav 1.5 and transiently transfected with wild-type (WT) or variant TCAP. Results:We identified two TCAP variants, c.145G>A:p.E49K and c.458G>A:p.R153H, in four individuals. p.E49K was found in two patients with ARVC or BrS. p.R153H was found in two patients with BrS or J-wave pattern ECG. No patient had variant hNav 1.5. Patch-clamp experiments demonstrated that peak sodium currents were significantly reduced in cells expressing p.R153H and p.E49K compared with WT-TCAP (66%, p.R153H; 72%, p.E49K). Voltage dependency of peak IV curve was rightward-shifted by 5 mV in cells expressing p.E49K compared with WT-TCAP. Voltage dependency of activation was not leftward-shifted by p.R153H, while voltage dependency of steady-state inactivation was leftward-shifted by p.E49K. Conclusions:We found two TCAP variants in the patients with BrS, J-wave pattern ECG, and ARVC that can cause loss-of-function of the hNav 1.5 in heterologous expression systems. Our observation suggests that these variants might impair INa and be associated with the patients' electrophysiological phenotypes. Further studies linking our experimental data to clinical phenotypes are warranted. |
| تدمد: | 1540-8159 0147-8389 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5f4a2fdadc78e51daec09bee3581e644 https://doi.org/10.1111/pace.13996 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....5f4a2fdadc78e51daec09bee3581e644 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15408159 01478389 |
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