Indole-3-carbinol inhibits the proliferation of colorectal carcinoma LoVo cells through activation of the apoptotic signaling pathway
| العنوان: | Indole-3-carbinol inhibits the proliferation of colorectal carcinoma LoVo cells through activation of the apoptotic signaling pathway |
|---|---|
| المؤلفون: | See-Hyoung Park, Myeong Jin Nam, Jae-Nam Lee, Hyung-Ho Lim, Chang-Min Lee |
| المصدر: | Human & Experimental Toxicology. 40:2099-2112 |
| بيانات النشر: | SAGE Publications, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Indoles, Health, Toxicology and Mutagenesis, Apoptosis, DNA Fragmentation, Toxicology, 03 medical and health sciences, 0302 clinical medicine, Annexin, Cell Line, Tumor, Humans, Cytotoxic T cell, Cell Proliferation, Chemistry, Cell Cycle, General Medicine, Cell cycle, Antineoplastic Agents, Phytogenic, Molecular biology, 030104 developmental biology, Cell culture, 030220 oncology & carcinogenesis, Cancer cell, DNA fragmentation, Apoptotic signaling pathway, Apoptosis Regulatory Proteins, Colorectal Neoplasms, Reactive Oxygen Species, Signal Transduction |
| الوصف: | Indole-3-carbinol (I3C) is a phytochemical that exhibits growth-inhibitory activity against various cancer cells. However, there are limited studies on the effects of I3C on colon cancer cells. In this study, the growth-inhibitory activity of I3C against the human colorectal carcinoma cell line (LoVo) was examined. The results of the 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide, colony formation, and cell counting assays revealed that I3C suppressed the proliferation of LoVo cells. Microscopy and wound-healing analyses revealed that I3C affected the morphology and inhibited the migration of LoVo cells, respectively. I3C induced apoptosis and DNA fragmentation as evidenced by the results of fluorescein isothiocyanate-conjugated annexin V staining and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling assay, respectively. Additionally, I3C arrested the cell cycle at the G0/G1 phase and enhanced the reactive oxygen species levels. Western blotting analysis revealed that treatment with I3C resulted in the activation of apoptotic proteins, such as poly(ADP-ribose) polymerase, caspase-3, caspase-7, caspase-9, Bax, Bim, and p53 in LoVo cells. These results indicate that I3C induces apoptosis in LoVo cells by upregulating p53, leading to the activation of Bax and caspases. Taken together, I3C exerts cytotoxic effects on LoVo cells by activating apoptosis. |
| تدمد: | 1477-0903 0960-3271 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5fed3533f099ed80dfccc4315973b1a4 https://doi.org/10.1177/09603271211021475 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....5fed3533f099ed80dfccc4315973b1a4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14770903 09603271 |
|---|