Age-Dependent Rat Lung Deposition Patterns of Inhaled 20 Nanometer Gold Nanoparticles and their Quantitative Biokinetics in Adult Rats
| العنوان: | Age-Dependent Rat Lung Deposition Patterns of Inhaled 20 Nanometer Gold Nanoparticles and their Quantitative Biokinetics in Adult Rats |
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| المؤلفون: | Martin Schäffler, Stephanie Hirn, Johannes C. Schittny, Neil Gibson, Winfried Möller, Wolfgang G. Kreyling, Carsten Schleh, Nadine Haberl, Alexander Wenk, Uwe Holzwarth |
| المصدر: | ACS Nano 12, 7771-7790 (2018) |
| بيانات النشر: | American Chemical Society (ACS), 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Pathology, medicine.medical_specialty, Mucociliary clearance, Metal Nanoparticles, General Physics and Astronomy, Spleen, 02 engineering and technology, Rats, Inbred WKY, Excretion, 03 medical and health sciences, Administration, Inhalation, medicine, Animals, Tissue Distribution, General Materials Science, Gold Nanoparticle Aerosols, Intratracheal Inhalation, Air-blood Barrier Translocation, Extrapulmonary Nanoparticle Accumulation, Nanoparticle Clearance, Alveolar Macrophage, Interstitial Nanoparticle Relocation, Epithelial Re-entrainment, Particle Size, 610 Medicine & health, Lung, Gastrointestinal tract, Inhalation, Age Factors, General Engineering, 021001 nanoscience & nanotechnology, Epithelium, Rats, Kinetics, 030104 developmental biology, medicine.anatomical_structure, Colloidal gold, Alveolar macrophage, 570 Life sciences, biology, Female, Gold, 0210 nano-technology |
| الوصف: | The increasing use of gold nanoparticles leads to a possible increase of exposure by inhalation. Therefore, we have studied the deposition patterns of inhaled 20 nm gold nanoparticles (AuNP) in 7-90 day old rats and their biokinetics in 60 day old ones. Wistar-Kyoto rats inhaled intratracheally 20 nm Au-195-radiolabeled AuNP by negative pressure ventilation over 2 h. Immediately afterward lungs were excised, inflated and microwave dried. AuNP deposition was analyzed by single-photon emission computed tomography, computed-tomography and autoradiography. Completely balanced, quantitative biodistributions in major organs and all body tissues and total excretion were analyzed from 1 h to 28 d after inhalation. Intratracheal inhalation caused AuNP deposition predominately in the caudal lungs, independent of age. About 30% AuNP were deposited on airway epithelia and rapidly cleared by mucociliary clearance. About 80% of AuNP deposited in alveoli was relocated from the epithelium into the interstitium within 24 h and was inaccessible to broncho-alveolar lavage. During interstitial long-term retention, re-entrainment within macrophages back onto the lung epithelium and to the larynx and gastrointestinal tract (GIT) dominated AuNP clearance (rate 0.03 d(-1)) In contrast, AuNP-translocation across the air-blood barrier was much smaller leading to persistent retention in secondary organs and tissues in the ranking order liver > soft issue > spleen > kidneys > skeleton > blood > uterus > heart > brain. The age independent, inhomogeneous AuNP deposition was probably caused by the negative pressure ventilation. Long-term AuNP clearance was dominated by macrophage-mediated transport from the interstitium to the larynx and GIT. Translocation across the rat air blood barrier appeared to be similar to that of humans for similar sized AuNP. |
| وصف الملف: | application/pdf |
| تدمد: | 1936-086X 1936-0851 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6054e2ffd75006841633a40927203f40 https://doi.org/10.1021/acsnano.8b01826 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....6054e2ffd75006841633a40927203f40 |
| قاعدة البيانات: | OpenAIRE |
Find this issue from the American Chemical Society | تدمد: | 1936086X 19360851 |
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