Human Peripheral Blood-Derived Endothelial Colony-Forming Cells Are Highly Similar to Mature Vascular Endothelial Cells yet Demonstrate a Transitional Transcriptomic Signature
| العنوان: | Human Peripheral Blood-Derived Endothelial Colony-Forming Cells Are Highly Similar to Mature Vascular Endothelial Cells yet Demonstrate a Transitional Transcriptomic Signature |
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| المؤلفون: | Marsel R. Kabilov, Daria K. Shishkova, Vera G. Matveeva, Anton G. Kutikhin, Alexey E. Tupikin, Larisa V. Antonova, E. A. Velikanova |
| المصدر: | Cells Volume 9 Issue 4 Cells, Vol 9, Iss 876, p 876 (2020) |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Male, Proteomics, 0301 basic medicine, CD31, 030204 cardiovascular system & hematology, Umbilical vein, Extracellular matrix, endothelial colony-forming cells, 0302 clinical medicine, endothelial lineages, lcsh:QH301-705.5, Tube formation, Principal Component Analysis, education.field_of_study, Stem Cells, Acetylation, Cell Differentiation, General Medicine, peripheral blood mononuclear cells, Coronary Vessels, Cell biology, Lipoproteins, LDL, adipose tissue-derived stromal vascular fraction, cardiovascular system, CD146, Population, Subcutaneous Fat, umbilical vein endothelial cells, Biology, Article, Fluorescence, Cell Line, 03 medical and health sciences, transcriptomic signatures, Human Umbilical Vein Endothelial Cells, Humans, education, Gene Expression Profiling, Lineage markers, Endothelial Cells, coronary artery endothelial cells, transcriptome profiling, Gene expression profiling, 030104 developmental biology, lcsh:Biology (General), Gene Expression Regulation, Leukocytes, Mononuclear, gene expression, Stromal Cells, RNA-seq, Transcriptome |
| الوصف: | Endothelial colony-forming cells (ECFC) are currently considered as a promising cell population for the pre-endothelialization or pre-vascularization of tissue-engineered constructs, including small-diameter biodegradable vascular grafts. However, the extent of heterogeneity between ECFC and mature vascular endothelial cells (EC) is unclear. Here, we performed a transcriptome-wide study to compare gene expression profiles of ECFC, human coronary artery endothelial cells (HCAEC), and human umbilical vein endothelial cells (HUVEC). Characterization of the abovementioned cell populations was carried out by immunophenotyping, tube formation assay, and evaluation of proliferation capability while global gene expression profiling was conducted by means of RNA-seq. ECFC were similar to HUVEC in terms of immunophenotype (CD31+vWF+KDR+CD146+CD34-CD133-CD45-CD90-) and tube formation activity yet had expectedly higher proliferative potential. HCAEC and HUVEC were generally similar to ECFC with regards to their global gene expression profile nevertheless, ECFC overexpressed specific markers of all endothelial lineages (NRP2, NOTCH4, LYVE1), in particular lymphatic EC (LYVE1), and had upregulated extracellular matrix and basement membrane genes (COL1A1, COL1A2, COL4A1, COL4A2). Proteomic profiling for endothelial lineage markers and angiogenic molecules generally confirmed RNA-seq results, indicating ECFC as an intermediate population between HCAEC and HUVEC. Therefore, gene expression profile and behavior of ECFC suggest their potential to be applied for a pre-endothelialization of bioartificial vascular grafts, whereas in terms of endothelial hierarchy they differ from HCAEC and HUVEC, having a transitional phenotype. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2073-4409 |
| DOI: | 10.3390/cells9040876 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6093fcc5196f72ce52f4dfd914e813be |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....6093fcc5196f72ce52f4dfd914e813be |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20734409 |
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| DOI: | 10.3390/cells9040876 |