Molecular epidemiology and virulence factors of methicillin‐resistantStaphylococcus aureusisolated from patients with bacteremia
| العنوان: | Molecular epidemiology and virulence factors of methicillin‐resistantStaphylococcus aureusisolated from patients with bacteremia |
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| المؤلفون: | Qute Choi, Sun Hoe Koo, Hyun-Jin Kim, Gye Cheol Kwon |
| المصدر: | Journal of Clinical Laboratory Analysis |
| بيانات النشر: | Wiley, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Male, Methicillin-Resistant Staphylococcus aureus, 0301 basic medicine, Microbiology (medical), Virulence Factors, medicine.drug_class, Clinical Biochemistry, Antibiotics, Virulence, Bacteremia, Microbial Sensitivity Tests, Biology, medicine.disease_cause, Microbiology, methicillin‐resistant Staphylococcus aureus, 03 medical and health sciences, 0302 clinical medicine, Antibiotic resistance, Pulsed-field gel electrophoresis, medicine, Humans, Immunology and Allergy, Phylogeny, Research Articles, Aged, Molecular Epidemiology, virulence gene, Molecular epidemiology, Biochemistry (medical), Public Health, Environmental and Occupational Health, Drug Resistance, Microbial, Hematology, bacterial infections and mycoses, medicine.disease, Methicillin-resistant Staphylococcus aureus, Electrophoresis, Gel, Pulsed-Field, ST5, Medical Laboratory Technology, Phenotype, 030104 developmental biology, 030220 oncology & carcinogenesis, Multilocus sequence typing, Female, ST72, Research Article |
| الوصف: | Background The various virulence factors of methicillin‐resistant Staphylococcus aureus bacteremia (MRSAB) are associated with a high mortality rate worldwide. Further studies are warranted to confirm the significant relationship between the strains and virulence genes. Here, we prospectively investigated the molecular characteristics underlying the genotypes and virulence factors of MRSA isolated from patients with bacteremia. Methods We collected 59 MRSA isolates from adult patients with bacteremia. Antimicrobial susceptibility results were obtained with the Vitek2 automated system. Genotypes were identified with multi‐locus sequence typing (MLST) and pulse‐field gel electrophoresis (PFGE), and 21 virulence genes were detected with polymerase chain reaction (PCR). Results The 59 MRSA isolates mainly comprised ST5 (n = 31, 52.5%) and ST72 (n = 22, 37.2%). Most ST5 isolates and all ST72 isolates were clustered into one and two PFGE groups, respectively. The mean number of virulence genes was higher in ST5 than in ST72. Sel was more frequently detected in ST5 than in ST72, whereas sec and sed were found only in ST5. ST5 had significantly higher resistance against many antibiotics than ST72. Conclusion Most MRSA isolates causing bacteremia were ST5 (CC5) and ST72 (CC8), and those belonging to the same STs were divided into only a few PFGE groups. ST5 was associated with higher antibiotic resistance and staphylococcal superantigen toxin genes, than ST72, which may be related to its higher virulence. |
| تدمد: | 1098-2825 0887-8013 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::60b506e6264d5439bf6e97debd89c1a2 https://doi.org/10.1002/jcla.23077 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....60b506e6264d5439bf6e97debd89c1a2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10982825 08878013 |
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