The unfolded protein response and endoplasmic reticulum protein targeting machineries converge on the stress sensor IRE1
| العنوان: | The unfolded protein response and endoplasmic reticulum protein targeting machineries converge on the stress sensor IRE1 |
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| المؤلفون: | Han Li, Peter Walter, Diego Acosta-Alvear, Florian Fröhlich, Tobias C. Walther, G. Elif Karagöz |
| المصدر: | eLife eLife, Vol 7 (2018) Acosta-Alvear, Diego; Karagöz, Gülsün Elif; Fröhlich, Florian; Li, Han; Walther, Tobias C; & Walter, Peter. (2018). The unfolded protein response and endoplasmic reticulum protein targeting machineries converge on the stress sensor IRE1.. eLife, 7. doi: 10.7554/elife.43036. UCSF: Retrieved from: http://www.escholarship.org/uc/item/1r05z55d |
| بيانات النشر: | eLife Sciences Publications, Ltd, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Protein Folding, medicine.disease_cause, Endoplasmic Reticulum, Ribosome, Protein targeting, cell biology, Signal recognition particle RNA, Biology (General), Chemistry, General Neuroscience, General Medicine, unfolded protein response, Protein-Serine-Threonine Kinases, Cell biology, Protein Transport, ribosome, Generic Health Relevance, Medicine, Protein folding, Eukaryotic Ribosome, Biotechnology, Research Article, Human, Protein Binding, QH301-705.5, Science, RNA-protein interactome, co-translational protein targeting, IRE1, Protein Serine-Threonine Kinases, General Biochemistry, Genetics and Molecular Biology, Vaccine Related, 03 medical and health sciences, Endoribonucleases, medicine, Genetics, Humans, human, General Immunology and Microbiology, Endoplasmic reticulum, RNA, Cell Biology, 030104 developmental biology, HEK293 Cells, Unfolded protein response, Biochemistry and Cell Biology, Ribosomes |
| الوصف: | The protein folding capacity of the endoplasmic reticulum (ER) is tightly regulated by a network of signaling pathways, known as the unfolded protein response (UPR). UPR sensors monitor the ER folding status to adjust ER folding capacity according to need. To understand how the UPR sensor IRE1 maintains ER homeostasis, we identified zero-length crosslinks of RNA to IRE1 with single nucleotide precision in vivo. We found that IRE1 specifically crosslinks to a subset of ER-targeted mRNAs, SRP RNA, ribosomal and transfer RNAs. Crosslink sites cluster in a discrete region of the ribosome surface spanning from the A-site to the polypeptide exit tunnel. Moreover, IRE1 binds to purified 80S ribosomes with high affinity, indicating association with ER-bound ribosomes. Our results suggest that the ER protein translocation and targeting machineries work together with the UPR to tune the ER’s protein folding load. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2050-084X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::60d1ad3585de045cfdb7044a12b90a6c http://europepmc.org/articles/PMC6336407 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....60d1ad3585de045cfdb7044a12b90a6c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 2050084X |
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