Intranasal Infection of Ferrets with SARS-CoV-2 as a Model for Asymptomatic Human Infection
| العنوان: | Intranasal Infection of Ferrets with SARS-CoV-2 as a Model for Asymptomatic Human Infection |
|---|---|
| المؤلفون: | Shelley G. Rhodes, Paul Skinner, Miles W. Carroll, Caroline J. Warren, Pauline M. van Diemen, Shellene Hurley, Samantha Watson, Fabian Z. X. Lean, Hugh Simmons, Helen Everett, Alexander M. P. Byrne, Ian H. Brown, Benjamin C. Mollett, Kevin R. Bewley, Kathryn A. Ryan, Sharon M. Brookes, Alejandro Núñez, Vivien J Coward, Yper Hall, Joe James |
| المصدر: | Viruses Viruses, Vol 13, Iss 113, p 113 (2021) Volume 13 Issue 1 |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Viral protein, viruses, lcsh:QR1-502, Y453F, Genome, Viral, Biology, medicine.disease_cause, Antibodies, Viral, Asymptomatic, lcsh:Microbiology, Article, 03 medical and health sciences, 0302 clinical medicine, Virology, medicine, Animals, 030212 general & internal medicine, Viral shedding, Respiratory system, ferret, Coronavirus, Subclinical infection, SARS-CoV-2, Ferrets, virus diseases, COVID-19, olfactory neuronal cells, Viral Load, 3. Good health, Virus Shedding, Disease Models, Animal, Nasal Mucosa, 030104 developmental biology, Infectious Diseases, medicine.anatomical_structure, Asymptomatic Diseases, Mutation, RNA, Viral, Female, medicine.symptom, Viral load, Respiratory tract |
| الوصف: | Ferrets were experimentally inoculated with SARS-CoV-2 (severe acute respiratory syndrome (SARS)-related coronavirus 2) to assess infection dynamics and host response. During the resulting subclinical infection, viral RNA was monitored between 2 and 21 days post-inoculation (dpi), and reached a peak in the upper respiratory cavity between 4 and 6 dpi. Viral genomic sequence analysis in samples from three animals identified the Y453F nucleotide substitution relative to the inoculum. Viral RNA was also detected in environmental samples, specifically in swabs of ferret fur. Microscopy analysis revealed viral protein and RNA in upper respiratory tract tissues, notably in cells of the respiratory and olfactory mucosae of the nasal turbinates, including olfactory neuronal cells. Antibody responses to the spike and nucleoprotein were detected from 21 dpi, but virus-neutralizing activity was low. A second intranasal inoculation (re-exposure) of two ferrets after a 17-day interval did not produce re-initiation of viral RNA shedding, but did amplify the humoral response in one animal. Therefore, ferrets can be experimentally infected with SARS-CoV-2 to model human asymptomatic infection. |
| وصف الملف: | application/pdf |
| تدمد: | 1999-4915 |
| DOI: | 10.3390/v13010113 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::610ca5d6d283a5c71b85e91bcca3e3bb |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....610ca5d6d283a5c71b85e91bcca3e3bb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19994915 |
|---|---|
| DOI: | 10.3390/v13010113 |