A Single Nucleotide Variant in the PPARγ-homolog Eip75B Affects Fecundity in Drosophila
| العنوان: | A Single Nucleotide Variant in the PPARγ-homolog Eip75B Affects Fecundity in Drosophila |
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| المؤلفون: | Katja M Hoedjes, Hristina Kostic, Thomas Flatt, Laurent Keller |
| المساهمون: | Ecology & Evolution |
| المصدر: | Molecular biology and evolution, 40(2):msad018, 1-12. Oxford University Press Hoedjes, K M, Kostic, H, Flatt, T & Keller, L 2023, ' A Single Nucleotide Variant in the PPARγ-homolog Eip75B Affects Fecundity in Drosophila ', Molecular biology and evolution, vol. 40, no. 2, msad018, pp. 1-12 . https://doi.org/10.1093/molbev/msad018 |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | reproduction, RNAi, aging, pleiotropy, Genetics, Drosophila, Molecular Biology, CRISPR/Cas9, Ecology, Evolution, Behavior and Systematics |
| الوصف: | Single nucleotide polymorphisms are the most common type of genetic variation, but how these variants contribute to the adaptation of complex phenotypes is largely unknown. Experimental evolution and genome-wide association studies have demonstrated that variation in the PPARγ-homolog Eip75B has associated with longevity and life-history differences in the fruit fly Drosophila melanogaster. Using RNAi knockdown, we first demonstrate that reduced expression of Eip75B in adult flies affects lifespan, egg-laying rate, and egg volume. We then tested the effects of a naturally occurring SNP within a cis-regulatory domain of Eip75B by applying two complementary approaches: a Mendelian randomization approach using lines of the Drosophila Genetic Reference Panel, and allelic replacement using precise CRISPR/Cas9-induced genome editing. Our experiments reveal that this natural polymorphism has a significant pleiotropic effect on fecundity and egg-to-adult viability, but not on longevity or other life-history traits. Our results provide a rare functional validation at the nucleotide level and identify a natural allelic variant affecting fitness and life-history adaptation. |
| اللغة: | English |
| تدمد: | 0737-4038 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6158372d6e7ed870b04adde5288aad0e https://research.vu.nl/en/publications/931533c3-19f0-43bc-8a5f-e238d34d0c00 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....6158372d6e7ed870b04adde5288aad0e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 07374038 |
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