Human Immunodeficiency Virus Type 2 Reverse Transcriptase Activity in Model Systems That Mimic Steps in Reverse Transcription
| العنوان: | Human Immunodeficiency Virus Type 2 Reverse Transcriptase Activity in Model Systems That Mimic Steps in Reverse Transcription |
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| المؤلفون: | Stuart F. J. Le Grice, Judith G. Levin, Jennifer T. Miller, Michael D. Powell, Kathryn J. Howard, Amnon Hizi, Klara Post, Jianhui Guo |
| المصدر: | Journal of Virology. 77:7623-7634 |
| بيانات النشر: | American Society for Microbiology, 2003. |
| سنة النشر: | 2003 |
| مصطلحات موضوعية: | DNA Replication, Transcription, Genetic, Ribonuclease H, Immunology, Replication, Microbiology, Virology, RNase H, Polymerase, DNA Primers, chemistry.chemical_classification, Base Sequence, biology, DNA synthesis, RNA-Directed DNA Polymerase, DNA replication, virus diseases, Molecular biology, HIV Reverse Transcriptase, Reverse transcriptase, Enzyme, chemistry, Insect Science, DNA, Viral, HIV-2, biology.protein, Primer (molecular biology) |
| الوصف: | Human immunodeficiency virus type 2 (HIV-2) infection is a serious problem in West Africa and Asia. However, there have been relatively few studies of HIV-2 reverse transcriptase (RT), a potential target for antiviral therapy. Detailed knowledge of HIV-2 RT activities is critical for development of specific high-throughput screening assays of potential inhibitors. Here, we have conducted a systematic evaluation of HIV-2 RT function, using assays that model specific steps in reverse transcription. Parallel studies were performed with HIV-1 RT. In general, under standard assay conditions, the polymerase and RNase H activities of the two enzymes were comparable. However, when the RT concentration was significantly reduced, HIV-2 RT was less active than the HIV-1 enzyme. HIV-2 RT was also impaired in its ability to catalyze secondary RNase H cleavage in assays that mimic tRNA primer removal during plus-strand transfer and degradation of genomic RNA fragments during minus-strand DNA synthesis. In addition, initiation of plus-strand DNA synthesis was much less efficient with HIV-2 RT than with HIV-1 RT. This may reflect architectural differences in the primer grip regions in the p66 (HIV-1) and p68 (HIV-2) palm subdomains of the two enzymes. The implications of our findings for antiviral therapy are discussed. |
| تدمد: | 1098-5514 0022-538X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::61b943452f77fd57139cb3a472a28dbc https://doi.org/10.1128/jvi.77.13.7623-7634.2003 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....61b943452f77fd57139cb3a472a28dbc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10985514 0022538X |
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