Cyclosporine A inhibits the expression of membrane type-I matrix metalloproteinase in gingiva
| العنوان: | Cyclosporine A inhibits the expression of membrane type-I matrix metalloproteinase in gingiva |
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| المؤلفون: | Hsien-Chung Chiu, Earl Fu, Gau Ch, Shin Nieh, Cheng-Yang Chiang, Yu-Tang Chin, Lu Yt, Hsiao-Pei Tu |
| المصدر: | Journal of Periodontal Research. 44:338-347 |
| بيانات النشر: | Wiley, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Male, Cell Survival, Gingiva, Gene Expression, Matrix Metalloproteinase Inhibitors, Matrix metalloproteinase, Rats, Sprague-Dawley, In vivo, Matrix Metalloproteinase 14, medicine, Animals, Humans, Protease Inhibitors, Zymography, RNA, Messenger, Fibroblast, Cells, Cultured, Tissue Inhibitor of Metalloproteinase-2, Metalloproteinase, Messenger RNA, Dose-Response Relationship, Drug, Gingival Overgrowth, Reverse Transcriptase Polymerase Chain Reaction, Chemistry, Fibroblasts, Molecular biology, In vitro, Rats, medicine.anatomical_structure, Biochemistry, Cyclosporine, Matrix Metalloproteinase 2, Periodontics, Immunohistochemistry, Electrophoresis, Polyacrylamide Gel, Matrix Metalloproteinase 1, Immunosuppressive Agents |
| الوصف: | Background and Objective: Membrane type-I matrix metalloproteinase (MMP) and tissue inhibitor of metalloproteinase-2 (TIMP-2) regulate the activation of MMP-2; however, their roles in the activation of MMP-2 in gingiva during treatment with cyclosporine A are still unknown. Therefore, the expressions of membrane type-I MMP and TIMP-2, as well as MMP-2, in gingivae upon treatment with cyclosporine A were examined in vivo and in vitro. Material and Methods: Thirty-four rats were divided into two groups after edentulous ridges were established. The experimental group received 30 mg/kg/d of cyclosporine A and the control group received vehicle. At the end of the experimental period, the rats were killed, the gingivae were obtained and the expression of mRNA and protein of membrane type-I MMP, TIMP-2 and MMP-2 in gingiva were examined using real-time polymerase chain reaction and immunohistochemistry. In human gingival fibroblasts, the activity of MMP-2 and the expression of MMP-2, membrane type-I MMP and TIMP-2 mRNAs were examined (using zymography and reverse transcription–polymerase chain reaction, respectively) after treatment with cyclosporine A. Results: In gingivae of rats, cyclosporine A significantly decreased the expression of mRNA and protein of membrane type-I MMP, but not of TIMP-2. The expression of MMP-2 mRNA was unaffected but the expression of MMP-2 protein showed a significant decrease upon treatment with cyclosporine A. In fibroblast culture medium, the presence of cyclosporine A induced a decrease in MMP-2 activity in a dose-dependent manner. The expression of MMP-2, membrane type-I MMP and TIMP-2 mRNAs in fibroblasts was not significantly affected by cyclosporine A; however, in fibroblasts the ratio of mRNA expression of membrane type-I MMP to that of TIMP-2 decreased as the cyclosporine A dose was increased. Conclusion: Cyclosporine A inhibits the expression of membrane type-I MMP in gingiva and it may further reduce the activation of MMP-2. |
| تدمد: | 1600-0765 0022-3484 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::61c7466b6b82f9750818d2a38e1989da https://doi.org/10.1111/j.1600-0765.2008.01126.x |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....61c7466b6b82f9750818d2a38e1989da |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16000765 00223484 |
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