DNA methylation of tumour necrosis factor (TNF) alpha gene is associated with specific blood fatty acid levels in a gender‐specific manner
| العنوان: | DNA methylation of tumour necrosis factor (TNF) alpha gene is associated with specific blood fatty acid levels in a gender‐specific manner |
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| المؤلفون: | Richard P Steel, Sarabjit S. Mastana, Boakye Gyimah, Bethan Hussey, James C. Reynolds, Martin R. Lindley, Ian M. Taylor |
| المصدر: | Molecular Genetics & Genomic Medicine, Vol 9, Iss 12, Pp n/a-n/a (2021) Molecular Genetics & Genomic Medicine |
| بيانات النشر: | Wiley, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Epigenomics, Male, 0301 basic medicine, TNF, 030105 genetics & heredity, QH426-470, Epigenesis, Genetic, chemistry.chemical_compound, Promoter Regions, Genetic, Genetics (clinical), chemistry.chemical_classification, DNA methylation, Fatty Acids, Methylation, Middle Aged, Docosahexaenoic acid, Saturated fatty acid, Female, Original Article, Arachidonic acid, Stearic acid, Polyunsaturated fatty acid, Adult, medicine.medical_specialty, Adolescent, Gas Chromatography-Mass Spectrometry, Young Adult, 03 medical and health sciences, Sex Factors, Internal medicine, medicine, arachidonic acid, Genetics, Humans, dry blood spots, Molecular Biology, docosahexaenoic Acid, Genetic Association Studies, Aged, Base Sequence, Tumor Necrosis Factor-alpha, Fatty acid, Sequence Analysis, DNA, Original Articles, 030104 developmental biology, Endocrinology, chemistry, inflammation, CpG Islands, Dried Blood Spot Testing |
| الوصف: | Background Fatty acids, specifically polyunsaturated fatty acids (PUFAs) play an important role in inflammation and its resolution, however, their interaction with the epigenome is relatively unexplored. Here we investigate the relationship between circulating blood fatty acids and the DNA methylation of the cytokine encoding gene tumour necrosis factor (TNF, OMIM 191160). Methods Using a cross‐sectional study approach, we collected blood samples from adults (N=88 (30 males, 58 females); 18–74 years old) for DNA methylation pyrosequencing analysis at four sites in TNF exon 1 and gas‐chromatography mass‐spectrometry analysis of the fatty acid profile of dried blood spots (DBS). Results Methylation levels of TNF exon 1 are significantly correlated with specific fatty acids in a gender‐specific manner. In the males the PUFAs Docosahexaenoic Acid (DHA) and Arachidonic Acid (AA) were positively associated with TNF methylation, as was the saturated fatty acid (SFA) Stearic Acid; in contrast, mono‐unsaturated fatty acids (MUFAs) had a negative association. In the females, omega‐6 PUFA γ‐Linolenic acid (GLA) was negatively correlated with TNF methylation; Adrenic acid and Eicosadienoic Acid were positively correlated with TNF methylation. Conclusion These results suggest that one way that fatty acids interact with the inflammation is through altered methylation profiles of cytokine genes; thus, providing potential therapeutic targets for nutritional and health interventions. Methylation levels of TNF exon 1 are significantly correlated with specific fatty acids in a gender‐specific manner. In the males the PUFAs Docosahexaenoic Acid (DHA) and Arachidonic Acid (AA) were positively associated. In females, omega‐6 PUFA γ‐Linolenic acid (GLA) was negatively correlated with TNF methylation. These results suggest that one way that fatty acids interact with the inflammation is through altered methylation profiles of cytokine genes; thus, providing potential therapeutic targets for nutritional and health interventions. |
| اللغة: | English |
| تدمد: | 2324-9269 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::61e6ebc477edbc7512bbad7b948a2c80 https://doaj.org/article/ecce07fcec4c4b7bb5c1b2a9c4dd6cca |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....61e6ebc477edbc7512bbad7b948a2c80 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23249269 |
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