Analysis of vertebrate vision in a 384-well imaging system
| العنوان: | Analysis of vertebrate vision in a 384-well imaging system |
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| المؤلفون: | Ruth M. Colwill, Robbert Creton, Elizabeth S. Kahn, Danielle E. Clift, Peter Baek, Tania M. Dominguez-Gonzalez, Kerry Eller, Renee J. Seto, Sara K. Tucker, Robert J. Thorn, Amanda Dombroski, Jason K. Sello |
| المصدر: | Scientific Reports, Vol 9, Iss 1, Pp 1-10 (2019) Scientific Reports |
| بيانات النشر: | Nature Publishing Group, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Visual perception, genetic structures, Computer science, Visual impairment, lcsh:Medicine, Article, Behavioural methods, 03 medical and health sciences, 0302 clinical medicine, Zebrafish larvae, medicine, Animals, Aminobenzoates, lcsh:Science, Beneficial effects, Swimming, Vision, Ocular, Zebrafish, Anesthetics, 030304 developmental biology, 0303 health sciences, Diazepam, Multidisciplinary, Behavior, Animal, lcsh:R, High-throughput screening, Single camera, Anti-Anxiety Agents, Visual function, Larva, Optomotor response, lcsh:Q, Visual system, medicine.symptom, Neuroscience, Software, 030217 neurology & neurosurgery |
| الوصف: | Visual impairment affects 253 million people worldwide and new approaches for prevention and treatment are urgently needed. While small molecules with potential beneficial effects can be examined in various model systems, the in vivo evaluation of visual function remains a challenge. The current study introduces a novel imaging system for measuring visually-guided behaviors in larval zebrafish. The imaging system is the first to image four 96-well plates with a single camera for automated measurements of activity in a 384-well format. In addition, it is the first system to project moving visual stimuli and analyze the optomotor response in the wells of a 96-well plate. We found that activity is affected by tricaine, diazepam and flumazenil. Surprisingly, diazepam treatments induce a loss of visual responses, at concentrations that do not affect activity or induce hyperactivity. Overall, our studies show that the developed imaging system is suitable for automated measurements of vertebrate vision in a high-throughput format. |
| اللغة: | English |
| تدمد: | 2045-2322 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::61eeb177971ff2712b3a4e2518d61de3 http://link.springer.com/article/10.1038/s41598-019-50372-0 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....61eeb177971ff2712b3a4e2518d61de3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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