Long noncoding RNA lnc-NAP sponges mmu-miR-139-5p to modulate Nanog functions in mouse ESCs and embryos

التفاصيل البيبلوغرافية
العنوان: Long noncoding RNA lnc-NAP sponges mmu-miR-139-5p to modulate Nanog functions in mouse ESCs and embryos
المؤلفون: Dongfang Xie, Tong, Man, Baolong Xia, Guihai Feng, Leyun Wang, Li, Ang, Guanzheng Luo, Haifeng Wan, Zeyu Zhang, Zhang, Hao, Yun-Gui Yang, Zhou, Qi, Wang, Meng, Wang, Xiu-Jie
بيانات النشر: Taylor & Francis, 2020.
سنة النشر: 2020
مصطلحات موضوعية: fungi, embryonic structures, biological phenomena, cell phenomena, and immunity, reproductive and urinary physiology
الوصف: The pluripotency of embryonic stem cells (ESCs) is controlled by a multilayer regulatory network, of which the key factors include core pluripotency genes Oct4, Sox2 and Nanog, and multiple microRNAs (miRNAs). Recently, long noncoding RNAs (lncRNAs) have been discovered as a class of new regulators for ESCs, and some lncRNAs could function as competing endogenous RNAs (ceRNAs) to regulate mRNAs by competitively binding to miRNAs. Here, we identify mmu-miR-139-5p as a new regulator for Nanog by targeting Nanog 3′ untranslated region (UTR) to repress Nanog expression in mouse ESCs and embryos. Such regulation could be released by an ESC-specifically expressed ceRNA named lnc-NAP. The expression of lnc-NAP is activated by OCT4, SOX2, as well as NANOG through promoter binding. Downregulation of lnc-NAP reduces Nanog abundance, which leads to decreased pluripotency of mouse ESCs and embryonic lethality. These results reveal lnc-NAP as a new regulator for Nanog in mouse ESCs, and uncover a feed-forward regulatory loop of Nanog through the participation of lnc-NAP.
DOI: 10.6084/m9.figshare.13134858.v1
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::621f4ba6eb5db3eced1543e22183b33d
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....621f4ba6eb5db3eced1543e22183b33d
قاعدة البيانات: OpenAIRE
الوصف
DOI:10.6084/m9.figshare.13134858.v1