Eimeria bovis infections induce G1 cell cycle arrest and a senescence-like phenotype in endothelial host cells
| العنوان: | Eimeria bovis infections induce G1 cell cycle arrest and a senescence-like phenotype in endothelial host cells |
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| المؤلفون: | Anja Taubert, Carolina Manosalva, Sara López-Osorio, Learta Pervizaj-Oruqaj, Zahady D. Velásquez, Carlos Hermosilla, Susanne Herold, Daniel Waiger |
| المصدر: | Parasitology |
| بيانات النشر: | Cambridge University Press (CUP), 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Senescence, 0303 health sciences, senescence, Cell cycle checkpoint, Intracellular parasite, Cell cycle, Biology, Apicomplexan parasites, 030308 mycology & parasitology, Cell biology, Endothelial stem cell, 03 medical and health sciences, Cyclin E1, Infectious Diseases, Eimeria bovis, cell cycle arrest, nucleolar condensation, endothelial cell, Animal Science and Zoology, Parasitology, Cyclin B1, G1 phase, Research Article, 030304 developmental biology |
| الوصف: | Apicomplexan parasites are well-known to modulate their host cells at diverse functional levels. As such, apicomplexan-induced alteration of host cellular cell cycle was described and appeared dependent on both, parasite species and host cell type. As a striking evidence of species-specific reactions, we here show that Eimeria bovis drives primary bovine umbilical vein endothelial cells (BUVECs) into a senescence-like phenotype during merogony I. In line with senescence characteristics, E. bovis induces a phenotypic change in host cell nuclei being characterized by nucleolar fusion and heterochromatin-enriched peripheries. By fibrillarin staining we confirm nucleoli sizes to be increased and their number per nucleus to be reduced in E. bovis-infected BUVECs. Additionally, nuclei of E. bovis-infected BUVECs showed enhanced signals for HH3K9me2 as heterochromatin marker thereby indicating an infection-induced change in heterochromatin transition. Furthermore, E. bovis-infected BUVECs show an enhanced β-galactosidase activity, which is a well-known marker of senescence. Referring to cell cycle progression, protein abundance profiles in E. bovis-infected endothelial cells revealed an up-regulation of cyclin E1 thereby indicating a cell cycle arrest at G1/S transition, signifying a senescence key feature. Similarly, abundance of G2 phase-specific cyclin B1 was found to be downregulated at the late phase of macromeront formation. Overall, these data indicate that the slow proliferative intracellular parasite E. bovis drives its host endothelial cells in a senescence-like status. So far, it remains to be elucidated whether this phenomenon indeed reflects an intentionally induced mechanism to profit from host cell-derived energy and metabolites present in a non-dividing cellular status. |
| تدمد: | 1469-8161 0031-1820 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6263b6903c8debe53796bfc2ba1c441f https://doi.org/10.1017/s0031182020002097 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....6263b6903c8debe53796bfc2ba1c441f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14698161 00311820 |
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