A diketopyrrolopyrrole-based ratiometric fluorescent probe for endogenous leucine aminopeptidase detecting and imaging with specific phototoxicity in tumor cells
| العنوان: | A diketopyrrolopyrrole-based ratiometric fluorescent probe for endogenous leucine aminopeptidase detecting and imaging with specific phototoxicity in tumor cells |
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| المؤلفون: | Chenyang Xu, Yu Wang, Jianli Hua, Jian Wang, Weibo Xu |
| المصدر: | Journal of Materials Chemistry B. 9:8842-8850 |
| بيانات النشر: | Royal Society of Chemistry (RSC), 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Cell, Biomedical Engineering, Antineoplastic Agents, Aminopeptidase, Leucyl Aminopeptidase, medicine, Humans, Pyrroles, General Materials Science, Photosensitizer, Cells, Cultured, Cell Proliferation, Fluorescent Dyes, Photosensitizing Agents, Chemistry, Optical Imaging, digestive, oral, and skin physiology, Proteolytic enzymes, General Chemistry, General Medicine, Hydrogen-Ion Concentration, Ketones, equipment and supplies, Fluorescence, medicine.anatomical_structure, Photochemotherapy, Biochemistry, Drug Screening Assays, Antitumor, Leucine, Reactive Oxygen Species, Phototoxicity, human activities, Fetal bovine serum |
| الوصف: | Leucine aminopeptidase (LAP) is a vital proteolytic enzyme, and its overexpression is often associated with many physiological diseases, such as liver dysfunction and breast cancer. Therefore, the accurate measurement of LAP concentrations in cells is critical for the diagnosis and prevention of related diseases. Herein, a new ratiometric fluorescent probe, DPP-Leu, based on diketopyrrolopyrrole (DPP) was designed and synthesized for LAP detection based on the specific enzymatic cleavage of the N-terminal leucine residue. The fluorescence intensity ratio of DPP-Leu (I548/I651) showed a remarkable change in the presence of LAP, with a limit of detection of 0.011 U L−1, and DPP-Leu was successfully applied to detect LAP in fetal bovine serum (FBS) and artificial urine. Cell imaging experiments revealed that DPP-Leu could target mitochondria and distinguish tumor cells with high LAP content from normal cells. Importantly, benefiting from the structural transformation of DPP-Leu to the photosensitizer 4 under LAP catalysis, the probe could kill tumor cells under light irradiation without damaging normal cells. |
| تدمد: | 2050-7518 2050-750X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::62a50e595783fded56a27ec064eea415 https://doi.org/10.1039/d1tb01480c |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....62a50e595783fded56a27ec064eea415 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20507518 2050750X |
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