A liposome-based ion release impedance sensor for biological detection
| العنوان: | A liposome-based ion release impedance sensor for biological detection |
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| المؤلفون: | Cartney E. Smith, Gregory L. Damhorst, Hengkan Ni, Magdalena M. Sobieraj, Eric Salm, Hyunjoon Kong, Rashid Bashir |
| المصدر: | Biomedical Microdevices. 15:895-905 |
| بيانات النشر: | Springer Science and Business Media LLC, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Ions, chemistry.chemical_classification, Ion release, Liposome, Materials science, 1,2-Dipalmitoylphosphatidylcholine, Biomolecule, Microfluidics, Biomedical Engineering, Impedance sensor, Nanotechnology, Biosensing Techniques, Microfluidic Analytical Techniques, Article, Dielectric spectroscopy, chemistry.chemical_compound, chemistry, Dielectric Spectroscopy, Dipalmitoylphosphatidylcholine, Liposomes, Viruses, Electric Impedance, Molecular Biology, Biosensor, Biomedical engineering |
| الوصف: | Low-cost detection of pathogens and biomolecules at the point-of-care promises to revolutionize medicine through more individualized monitoring and increased accessibility to diagnostics in remote and resource-limited areas. While many approaches to biosensing are still limited by expensive components or inadequate portability, we present here an ELISA-inspired lab-on-a-chip strategy for biological detection based on liposome tagging and ion-release impedance spectroscopy. Ion-encapsulating dipalmitoylphosphatidylcholine (DPPC) liposomes can be functionalized with antibodies and are stable in deionized water yet permeabilized for ion release upon heating, making them ideal reporters for electrical biosensing of surface-immobilized antigens. We demonstrate the quantification of these liposomes by real-time impedance measurements, as well as the qualitative detection of viruses as a proof-of-concept toward a portable platform for viral load determination which can be applied broadly to the detection of pathogens and other biomolecules. |
| تدمد: | 1572-8781 1387-2176 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::62a6ccfe9362a49fc9a6a921e6943382 https://doi.org/10.1007/s10544-013-9778-4 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....62a6ccfe9362a49fc9a6a921e6943382 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 15728781 13872176 |
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