Differential Effects of Long Term FTY720 Treatment on Endothelial versus Smooth Muscle Cell Signaling to S1P in Rat Mesenteric Arteries
| العنوان: | Differential Effects of Long Term FTY720 Treatment on Endothelial versus Smooth Muscle Cell Signaling to S1P in Rat Mesenteric Arteries |
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| المؤلفون: | Leo E. Deelman, S. P. H. Lambooy, Hendrik Buikema, Jacob van den Born, Robert H. Henning, Saleh Yazdani, Mahdi Hamidi Shishavan, Arash Bidadkosh |
| المساهمون: | Groningen Kidney Center (GKC), Groningen Institute for Organ Transplantation (GIOT) |
| المصدر: | PLoS ONE, 11(9):e0162029. PUBLIC LIBRARY SCIENCE PLoS ONE PLoS ONE, Vol 11, Iss 9, p e0162029 (2016) |
| بيانات النشر: | PUBLIC LIBRARY SCIENCE, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Muscle Physiology, Time Factors, Physiology, lcsh:Medicine, Hemodynamics, Blood Pressure, Muscle Development, Vascular Medicine, Epithelium, Animal Cells, Sphingosine, hemic and lymphatic diseases, VASCULATURE, Medicine and Health Sciences, lcsh:Science, Receptor, Musculoskeletal System, Mesenteric arteries, Smooth Muscles, Multidisciplinary, biology, Muscles, Arteries, Mesenteric Arteries, Receptors, Lysosphingolipid, medicine.anatomical_structure, HEART, lipids (amino acids, peptides, and proteins), Anatomy, Cellular Types, medicine.symptom, Perfusion, Research Article, Muscle Contraction, Signal Transduction, medicine.medical_specialty, Endothelium, Myocytes, Smooth Muscle, INHIBITION, Constriction, 03 medical and health sciences, Renal Arteries, Internal medicine, Pressure, medicine, Animals, Humans, CONSTRICTION, Rats, Wistar, MODULATOR, Fingolimod Hydrochloride, lcsh:R, Body Weight, Biology and Life Sciences, Endothelial Cells, Epithelial Cells, Cell Biology, SPHINGOSINE 1-PHOSPHATE RECEPTORS, Rats, SPHINGOSINE-1-PHOSPHATE RECEPTOR-2, Biological Tissue, 030104 developmental biology, Endocrinology, Gene Expression Regulation, Cardiovascular Anatomy, biology.protein, Blood Vessels, lcsh:Q, Cyclooxygenase, Lysophospholipids, COX-2 EXPRESSION, VASOCONSTRICTION, Vasoconstriction, RESISTANCE |
| الوصف: | The sphingosine-1-phosphate (S1P) analog FTY720 exerts pleiotropic effects on the cardiovascular system and causes down-regulation of S1P receptors. Myogenic constriction is an important mechanism regulating resistance vessel function and is known to be modulated by S1P. Here we investigated myogenic constriction and vascular function of mesenteric arteries of rats chronically treated with FTY720. Wistar rats received FTY720 1mg/kg/daily for six weeks. At termination, blood pressure was recorded and small mesenteric arteries collected for vascular studies in a perfusion set up. Myogenic constriction to increased intraluminal pressure was low, but a sub-threshold dose of S1P profoundly augmented myogenic constriction in arteries of both controls and animals chronically treated with FTY720. Interestingly, endothelial denudation blocked the response to S1P in arteries of FTY720-treated animals, but not in control rats. In acute experiments, presence of FTY720 significantly augmented the contractile response to S1P, an effect that was partially abolished after the inhibition of cyclooxygenase (COX-)-derived prostaglandins. FTY720 down regulated S1P1 but not S1P2 in renal resistance arteries and in cultured human endothelial cells. This study therefore demonstrates the endothelium is able to compensate for the complete loss of responsiveness of the smooth muscle layer to S1P after long term FTY720 treatment through a mechanism that most likely involves enhanced production of contractile prostaglandins by the endothelium. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::62fc76afd431c718958fa6a14c4e80ec https://research.rug.nl/en/publications/efbffddd-1060-405c-92e2-5711c7f80369 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....62fc76afd431c718958fa6a14c4e80ec |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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