Hybrid Gene Origination Creates Human-Virus Chimeric Proteins during Infection
| العنوان: | Hybrid Gene Origination Creates Human-Virus Chimeric Proteins during Infection |
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| المؤلفون: | Jiajie Wei, Paul Digard, Robert J. Gifford, Nerea Irigoyen, Megan K. L. MacLeod, Alessandro Vannini, James P. Gibbs, Laura Campisi, Brad R. Rosenberg, Joshua D. Jones, Max W. Chang, Cheng Huang, Quan Gu, Edward C. Hutchinson, Veronica V. Rezelj, Christopher Benner, Helen M. Wise, Jessica Sook Yuin Ho, Jeffrey R. Johnson, Guojun Wang, Matthew Angel, Yesai Fstkchyan, Slobodan Paessler, Robyn M. Kaake, Nan Zhao, Maria João Amorim, Marta Alenquer, Elizabeth Sloan, Sara Clohisey, Ingeborg van Knippenberg, Harm van Bakel, Simin Zheng, Nevan J. Krogan, Liliane Chung, Adam M. Dinan, Bo Wang, Benjamin Greenbaum, Léa Meyer, Natasha Moshkina, Ian Brierley, Zeyu Zhu, Zuleyma Peralta, Adolfo García-Sastre, Andrew E. Firth, Marta Łuksza, Emily R. Miraldi, Vladimir Roudko, Ivan Marazzi, Rong Shen, Carles Martínez-Romero, Yixuan Ma, Jonathan W. Yewdell, J Kenneth Baillie, Justine Noel |
| المساهمون: | Dinan, Adam [0000-0003-2812-1616], Irigoyen, Nerea [0000-0001-6346-3369], Brierley, Ian [0000-0003-3965-4370], Firth, Andrew [0000-0002-7986-9520], Apollo - University of Cambridge Repository |
| المصدر: | Yuin Ho, J S, Angel, M, Ma, Y, Sloan, E, Wang, G, Martinez-Romero, C, Alenquer, M, Roudko, V, Chung, L, Zheng, S, Chang, M, Fstkchyan, Y, Clohisey, S, Dinan, A M, Gibbs, J, Gifford, R, Shen, R, Gu, Q, Irigoyen, N, Campisi, L, Huang, C, Zhao, N, Jones, J, van Knippenberg, I, Zhu, Z, Moshkina, N, Meyer, L, Noel, J, Peralta, Z, Rezelji, V, Kaake, R, Rosenberg, B, Wang, B, Wei, J, Paessler, S, Wise, H, Johnson, J, Vannini, A, Amorim, M J, Baillie, K, Miraldi, E R, Benner, C, Brierley, I, Digard, P, Luksza, M, Firth, A E, Krogan, N J, Greenbaum, B D, MacLeod, M K, van Bakel, H, García-Sastre, A, Yewdell, J W, Hutchinson, E & Marrazi, I 2020, ' Hybrid Gene Origination Creates Human-Virus Chimeric Protein During Infection ', Cell, vol. 181, no. 7, pp. 1502-1517 . https://doi.org/10.1016/j.cell.2020.05.035 Cell |
| بيانات النشر: | Elsevier BV, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Transcription, Genetic, viruses, Mutant Chimeric Proteins, medicine.disease_cause, Virus Replication, gene origination, segmented negative-strand RNA viruses, viral evolution, Mice, 0302 clinical medicine, RNA Virus Infections, Cricetinae, RNA hybrid, Genetics, 0303 health sciences, 3. Good health, Influenza A virus, Viral evolution, RNA, Viral, influenza, RNA Caps, Recombinant Fusion Proteins, Biology, General Biochemistry, Genetics and Molecular Biology, Cap snatching, Cell Line, 03 medical and health sciences, Open Reading Frames, Viral Proteins, Dogs, chimeric proteins, Plant virus, upstream AUG, medicine, uORFs, Animals, Humans, RNA Viruses, RNA, Messenger, Gene, 030304 developmental biology, RNA, cap-snatching, RNA-Dependent RNA Polymerase, Fusion protein, viral RNA, Lassa virus, Cattle, Human Virus, 5' Untranslated Regions, 030217 neurology & neurosurgery |
| الوصف: | RNA viruses are a major human health threat. The life cycles of many highly pathogenic RNA viruses like influenza A virus (IAV) and Lassa virus depends on host mRNA, as viral polymerases cleave 5’-m7G-capped host transcripts to primeviral mRNA synthesis (‘cap-snatching’). We hypothesized that start codons within cap-snatched host transcripts could generate chimeric human-viral mRNAs with coding potential. We report the existence of this mechanism of gene origination, that we named ‘start-snatching’. Depending on the reading frame, start-snatching allows the translation of host and viral “untranslated regions” (UTRs) to create Nterminallyextended viral proteins or entirely novel polypeptides by geneticoverprinting. We show that both types of chimeric proteins are made in IAVinfectedcells, generate T cell responses and contribute to virulence. Our resultsindicate that during infection with IAV, and likely a multitude of other human-,animal- and plant-viruses, a host-dependent mechanism allows the genesis ofhybrid genes. |
| وصف الملف: | application/pdf; PDF |
| تدمد: | 0092-8674 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::63665551fab0a3b9b7b770a82f00e7e2 https://www.repository.cam.ac.uk/handle/1810/307677 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....63665551fab0a3b9b7b770a82f00e7e2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00928674 |
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