Evaluation of bioaerosol samplers for the detection and quantification of influenza virus from artificial aerosols and influenza virus–infected ferrets
| العنوان: | Evaluation of bioaerosol samplers for the detection and quantification of influenza virus from artificial aerosols and influenza virus–infected ferrets |
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| المؤلفون: | Christian Bekking, Nicolas Groulx, Mairead Finn, Samira Mubareka, Nathan Doggett, Lily Yip |
| المصدر: | Influenza and Other Respiratory Viruses |
| بيانات النشر: | Wiley, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Pulmonary and Respiratory Medicine, Epidemiology, Indoor bioaerosol, Air Microbiology, 030312 virology, influenza virus, Virus, Specimen Handling, 03 medical and health sciences, Orthomyxoviridae Infections, Animals, Viral rna, Particle Size, Infectious virus, Aerosols, 0303 health sciences, Anti-Inflammatory Agents, Non-Steroidal, Ferrets, Public Health, Environmental and Occupational Health, Humidity, Original Articles, Device use, Virology, Disease Models, Animal, Infectious Diseases, bioaerosols, Influenza virus RNA, Influenza A virus, 13. Climate action, RNA, Viral, Respiratory virus, Environmental science, Original Article, bioaerosol samplers, ferret model, Bioaerosol |
| الوصف: | Background Bioaerosol sampling devices are necessary for the characterization of infectious bioaerosols emitted by naturally-infected hosts with acute respiratory virus infections. Assessment of these devices under multiple experimental conditions will provide insight for device use. Objectives The primary objective of this study was to assess and compare bioaerosol sampling devices using a) an in vitro, environmentally-controlled artificial bioaerosol system at a range of different RH conditions and b) an in vivo bioaerosol system of influenza virus-infected ferrets under controlled environmental conditions. Secondarily, we also sought to examine the impact of NSAIDs on bioaerosol emission in influenza virus-infected ferrets to address its potential as a determinant of bioaerosol emission. Methods We examined the performance of low and moderate volume bioaerosol samplers for the collection of viral RNA and infectious influenza virus in vitroand in vivo using artificial bioaerosols and the ferret model of influenza virus infection. The following samplers were tested: the polytetrafluoroethylene filter (PTFE filter), the 2-stage National Institute of Occupational Safety and Health cyclone sampler (NIOSH cyclone sampler) and the 6-stage viable Andersen impactor (Andersen impactor). Results The PTFE filter and NIOSH cyclone sampler collected similar amounts of viral RNA and infectious virus from artificially-generated aerosols under a range of relative humidities (RH). Using the ferret model, the PTFE filter, NIOSH cyclone sampler and the Andersen impactor collected up to 3.66 log10 copies of RNA/L air, 3.84 log10 copies of RNA/L air and 6.09 log10 copies of RNA/L air respectively at peak recovery. Infectious virus was recovered from the PTFE filter and NIOSH cyclone samplers on the peak day of viral RNA recovery. Conclusion The PTFE filter and NIOSH cyclone sampler are useful for influenza virus RNA and infectious virus collection and may be considered for clinical and environmental settings. |
| تدمد: | 1750-2659 1750-2640 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::637b5055bffd592e66d9dc7ed4d80cd3 https://doi.org/10.1111/irv.12678 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....637b5055bffd592e66d9dc7ed4d80cd3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17502659 17502640 |
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