Inactivation of the Glial Fibrillary Acidic Protein Gene, But Not That of Vimentin, Improves Neuronal Survival and Neurite Growth by Modifying Adhesion Molecule Expression
| العنوان: | Inactivation of the Glial Fibrillary Acidic Protein Gene, But Not That of Vimentin, Improves Neuronal Survival and Neurite Growth by Modifying Adhesion Molecule Expression |
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| المؤلفون: | Julie Lannoy, Véronique Menet, Emma Colucci-Guyon, Minerva Giménez y Ribotta, Marie Jeanne Drian, Alain Privat, Norbert Chauvet |
| المصدر: | The Journal of Neuroscience. 21:6147-6158 |
| بيانات النشر: | Society for Neuroscience, 2001. |
| سنة النشر: | 2001 |
| مصطلحات موضوعية: | Cell Survival, Intermediate Filaments, Cell Count, Mice, Inbred Strains, Nerve Tissue Proteins, Vimentin, macromolecular substances, Models, Biological, Glial scar, Nestin, Extracellular matrix, Mice, Intermediate Filament Proteins, Downregulation and upregulation, Glial Fibrillary Acidic Protein, Cell Adhesion, Neurites, Animals, ARTICLE, Cytoskeleton, Intermediate filament, Neural Cell Adhesion Molecules, Cells, Cultured, Mice, Knockout, Neurons, Glial fibrillary acidic protein, biology, Cell adhesion molecule, General Neuroscience, Cadherins, Coculture Techniques, Extracellular Matrix, Fibronectins, Cell biology, nervous system, Astrocytes, Gene Targeting, biology.protein |
| الوصف: | Intermediate filaments (IFs) are a major component of the cytoskeleton in astrocytes. Their role is far from being completely understood. Immature astrocytes play a major role in neuronal migration and neuritogenesis, and their IFs are mainly composed of vimentin. In mature differentiated astrocytes, vimentin is replaced by the IF protein glial fibrillary acidic protein (GFAP). In response to injury of the CNS in the adult, astrocytes become reactive, upregulate the expression of GFAP, and reexpress vimentin. These modifications contribute to the formation of a glial scar that is obstructive to axonal regeneration. Nevertheless, astrocytes in vitro are considered to be the ideal substratum for the growth of embryonic CNS axons. In the present study, we have examined the potential role of these two major IF proteins in both neuronal survival and neurite growth. For this purpose, we cocultured wild-type neurons on astrocytes from three types of knock-out (KO) mice for GFAP or/and vimentin in a neuron-astrocyte coculture model. We show that the double KO astrocytes present many features of immaturity and greatly improve survival and neurite growth of cocultured neurons by increasing cell-cell contact and secreting diffusible factors. Moreover, our data suggest that the absence of vimentin is not a key element in the permissivity of the mutant astrocytes. Finally, we show that only the absence of GFAP is associated with an increased expression of some extracellular matrix and adhesion molecules. To conclude, our results suggest that GFAP expression is able to modulate key biochemical properties of astrocytes that are implicated in their permissivity. |
| تدمد: | 1529-2401 0270-6474 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::63b693fa9c07757c3159aa4307a49db3 https://doi.org/10.1523/jneurosci.21-16-06147.2001 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....63b693fa9c07757c3159aa4307a49db3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15292401 02706474 |
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