Chloroplasts alter their morphology and accumulate at the pathogen interface during infection by Phytophthora infestans
| العنوان: | Chloroplasts alter their morphology and accumulate at the pathogen interface during infection by Phytophthora infestans |
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| المؤلفون: | Alexandre Y Leary, Benji C. Bateman, David C. A. Gaboriau, Stanley W. Botchway, Virendrasinh Khandare, Zachary Savage, Imogen Sparkes, Yasin Tumtas, Indranil Pan, Andrew D. Ward, Martin H. Schattat, Tolga O. Bozkurt, Yuxi Liang, Lok Him Yuen, Pooja Pandey, Cian Duggan, María Eugenia Segretin, Alexia Toufexi |
| المساهمون: | Biotechnology and Biological Sciences Research Council (BBSRC), Biotechnology and Biological Sciences Research Council |
| المصدر: | Savage, Z, Duggan, C, Toufexi, A, Pandey, P, Liang, Y, Eugenia Segretin, M, Him Yuen, L, Gaboriau, D C A, Leary, A Y, Tumtas, Y, Khandare, V, Ward, A D, Botchway, S W, Bateman, B C, Pan, I, Schattat, M, Sparkes, I & Bozkurt, T O 2021, ' Chloroplasts alter their morphology and accumulate at the pathogen interface during infection by Phytophthora infestans ', Plant Journal, vol. 107, no. 6, pp. 1771-1787 . https://doi.org/10.1111/tpj.15416 CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| بيانات النشر: | Society for Experimental Biology, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0106 biological sciences, chloroplast movement, Chloroplasts, Light, Optical Tweezers, PROTEIN, Plant Science, SUSCEPTIBILITY, 0601 Biochemistry and Cell Biology, 01 natural sciences, purl.org/becyt/ford/1 [https], laser capture, Haustorium, Plant Immunity, Pathogen, 2. Zero hunger, 0303 health sciences, Microscopy, Confocal, Chemistry, Effector, food and beverages, focalimmunity, Plants, Genetically Modified, ARABIDOPSIS, Cell biology, Chloroplast, Actin Cytoskeleton, Dinitrobenzenes, MICROTUBULES, haustorium, STROMULES, Host-Pathogen Interactions, Thiazolidines, Life Sciences & Biomedicine, effectors, Stromule, Phytophthora infestans, Plant Biology & Botany, ENDOPLASMIC-RETICULUM, 0607 Plant Biology, Biology, stromule, 03 medical and health sciences, MOVEMENT, Organelle, Sulfanilamides, Tobacco, Genetics, focal immunity, PLANT, purl.org/becyt/ford/1.6 [https], Actin, 030304 developmental biology, Plant Diseases, Science & Technology, Plant Sciences, Cell Biology, Actin cytoskeleton, Bridged Bicyclo Compounds, Heterocyclic, FOCAL IMMUNITY, Plant Leaves, MEMBRANE, Reactive Oxygen Species, APOPLASTIC EFFECTORS, 010606 plant biology & botany |
| الوصف: | Upon immune activation, chloroplasts switch off photosynthesis, produce antimicrobial compounds and associate with the nucleus through tubular extensions called stromules. Although it is well established that chloroplasts alter their position in response to light, little is known about the dynamics of chloroplast movement in response to pathogen attack. Here, we report that during infection with the Irish potato famine pathogen Phytophthora infestans, chloroplasts accumulate at the pathogen interface, associating with the specialized membrane that engulfs the pathogen haustorium. The chemical inhibition of actin polymerization reduces the accumulation of chloroplasts at pathogen haustoria, suggesting that this process is partially dependent on the actin cytoskeleton. However, chloroplast accumulation at haustoria does not necessarily rely on movement of the nucleus to this interface and is not affected by light conditions. Stromules are typically induced during infection, embracing haustoria and facilitating chloroplast interactions, to form dynamic organelle clusters. We found that infection-triggered stromule formation relies on BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED KINASE 1 (BAK1)-mediated surface immune signaling, whereas chloroplast repositioning towards haustoria does not. Consistent with the defense-related induction of stromules, effector-mediated suppression of BAK1-mediated immune signaling reduced stromule formation during infection. On the other hand, immune recognition of the same effector stimulated stromules, presumably via a different pathway. These findings implicate chloroplasts in a polarized response upon pathogen attack and point to more complex functions of these organelles in plant–pathogen interactions. Fil: Savage, Zachary. Imperial College London; Reino Unido Fil: Duggan, Cian. Imperial College London; Reino Unido Fil: Toufexi, Alexia. Imperial College London; Reino Unido Fil: Pandey, Pooja. Imperial College London; Reino Unido Fil: Liang, Yuxi. Imperial College London; Reino Unido Fil: Segretin, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina Fil: Yuen, Lok Him. Imperial College London; Reino Unido Fil: Gaboriau, David C. A.. Imperial College London; Reino Unido Fil: Leary, Alexandre Y.. Imperial College London; Reino Unido Fil: Tumtas, Yasin. Imperial College London; Reino Unido Fil: Khandare, Virendrasinh. Imperial College London; Reino Unido Fil: Ward, Andrew D.. Science and Technology Facilities Council; Reino Unido Fil: Botchway, Stanley W.. Science and Technology Facilities Council; Reino Unido Fil: Bateman, Benji C.. Science and Technology Facilities Council; Reino Unido Fil: Pan, Indranil. Alan Turing Institute; Reino Unido. Imperial College London; Reino Unido Fil: Schattat, Martin. Martin Luther Universitat Halle-Wittenberg; Alemania Fil: Sparkes, Imogen. University of Bristol; Reino Unido Fil: Bozkurt, Osman Tolga. Imperial College London; Reino Unido |
| وصف الملف: | application/pdf |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::64010d10c4e4cb80b04fdfc547b93452 http://hdl.handle.net/10044/1/90860 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....64010d10c4e4cb80b04fdfc547b93452 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |