The MicroRNA hsa-let-7g Promotes Proliferation and Inhibits Apoptosis in Lung Cancer by Targeting HOXB1
| العنوان: | The MicroRNA hsa-let-7g Promotes Proliferation and Inhibits Apoptosis in Lung Cancer by Targeting HOXB1 |
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| المؤلفون: | Kuang Xiao, Qian Zhou, Shengwei Ma, Fenghe Cui |
| المصدر: | Yonsei Medical Journal |
| بيانات النشر: | Yonsei University College of Medicine, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Lung Neoplasms, animal structures, Cell Survival, proliferation, Down-Regulation, Treatment of lung cancer, 030204 cardiovascular system & hematology, 03 medical and health sciences, 0302 clinical medicine, Downregulation and upregulation, Cell Line, Tumor, microRNA, medicine, Humans, Gene Silencing, RNA, Messenger, Viability assay, Lung cancer, miRNA, Cell Proliferation, Homeodomain Proteins, Cell growth, Chemistry, apoptosis, General Medicine, Transfection, hsa-let-7g, medicine.disease, Gene Expression Regulation, Neoplastic, body regions, MicroRNAs, Oncology, Apoptosis, 030220 oncology & carcinogenesis, embryonic structures, Cancer research, Original Article, HOXB1 |
| الوصف: | Purpose The goal of this study was to explore the effects of hsa-let-7g on cell proliferation and apoptosis, and elucidate its role in lung cancer development. Materials and Methods The expression levels of has-let-7g and HOXB1 in tissues and cells were measured by qRT-PCR. An inhibitor of hsa-let-7g or one targeting a control messenger RNA were transfected into A549 and H1944 lung cancer cells, and the effects of hsa-let-7g dysregulation on cell viability and apoptosis were analyzed using CCK-8 and apoptosis detection assays. HOXB1 was confirmed as the target gene of hsa-let-7g, based on luciferase reporter assay results. The relationship between hsa-let-7g and HOXB1 was confirmed by co-transfection of inhibitors of hsa-let-7g and HOXB1 followed by Western blot, CCK-8, and apoptosis detection assays. Results We observed high expression of hsa-let-7g in lung cancer tissues compared to the corresponding normal tissues, and generally higher expression of hsa-let-7g in patients with advanced tumor classification. The results of CCK-8 and apoptosis detection experiments showed that the inhibition of hsa-let-7g significantly inhibited proliferation of A549 and H1944 cells, but also promoted apoptosis. HOXB1 is a specific target of hsa-let-7g, and downregulation of HOXB1 in lung cancer cells reversed the suppressive effects caused by knocking down hsa-let-7g. Conclusion These data collectively suggest that the expression of hsa-let-7g inhibits lung cancer cells apoptosis and promotes proliferation by down-regulating HOXB1. The results from this study demonstrate the potential of hsa-let-7g/HOXB1 axis as a therapeutic target for the treatment of lung cancer. |
| تدمد: | 1976-2437 0513-5796 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::64140f44c8630051ff35775322a6b9af https://doi.org/10.3349/ymj.2020.61.3.210 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....64140f44c8630051ff35775322a6b9af |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19762437 05135796 |
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