Rabbit Monoclonal Antibody Specifically Recognizing a Linear Epitope in the RBD of SARS-CoV-2 Spike Protein
| العنوان: | Rabbit Monoclonal Antibody Specifically Recognizing a Linear Epitope in the RBD of SARS-CoV-2 Spike Protein |
|---|---|
| المؤلفون: | Ningshao Xia, Junyu Chen, Yixin Chen, Junping Hong, Yingbin Wang, Wu Qian, Xijing Wang, Qian Wang |
| المصدر: | Vaccines, Vol 9, Iss 829, p 829 (2021) Vaccines Volume 9 Issue 8 |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, medicine.drug_class, Immunology, linear B-cell epitope, Monoclonal antibody, spike protein, Peripheral blood mononuclear cell, Article, RBD, 03 medical and health sciences, 0302 clinical medicine, Western blot, rabbit monoclonal antibody, Viral entry, Drug Discovery, medicine, Pharmacology (medical), B cell, Pharmacology, biology, medicine.diagnostic_test, Linear epitope, SARS-CoV-2, Virology, 030104 developmental biology, Infectious Diseases, medicine.anatomical_structure, Polyclonal antibodies, 030220 oncology & carcinogenesis, biology.protein, Immunohistochemistry, Medicine, IHC |
| الوصف: | To date, SARS-CoV-2 pandemic has caused more than 188 million infections and 4.06 million deaths worldwide. The receptor-binding domain (RBD) of the SARS-CoV-2 spike protein has been regarded as an important target for vaccine and therapeutics development because it plays a key role in binding the human cell receptor ACE2 that is required for viral entry. However, it is not easy to detect RBD in Western blot using polyclonal antibody, suggesting that RBD may form a complicated conformation under native condition and bear rare linear epitope. So far, no linear epitope on RBD is reported. Thus, a monoclonal antibody (mAb) that recognizes linear epitope on RBD will become valuable. In the present study, an RBD-specific rabbit antibody named 9E1 was isolated from peripheral blood mononuclear cells (PBMC) of immunized rabbit by RBD-specific single B cell sorting and mapped to a highly conserved linear epitope within twelve amino acids 480CNGVEGFNCYFP491 on RBD. 9E1 works well in Western blot on S protein and immunohistochemistry on the SARS-CoV-2 infected tissue sections. The results demonstrated that 9E1 can be used as a useful tool for pathological and functional studies of SARS-CoV-2. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::641813d56e96bf0a83a3c4cbb11a8454 https://www.mdpi.com/2076-393X/9/8/829 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....641813d56e96bf0a83a3c4cbb11a8454 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |