Isolation and Characterization of Environmental Bacteria Capable of Extracellular Biosorption of Mercury
| العنوان: | Isolation and Characterization of Environmental Bacteria Capable of Extracellular Biosorption of Mercury |
|---|---|
| المؤلفون: | Séverine Zirah, Carine Lombard, Jean-Michel Guigner, Paul Soreau, Florence Brian-Jaisson, Manon Vandervennet, Fabienne François, Jean Peduzzi, Grégory Martino, Anne-Laure Molinier, Sylvie Rebuffat, Daniel Garcia, David Pignol |
| المساهمون: | Molécules de Communication et Adaptation des Micro-organismes (MCAM), Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS), Institut de minéralogie, de physique des matériaux et de cosmochimie (IMPMC), Muséum national d'Histoire naturelle (MNHN)-Institut de recherche pour le développement [IRD] : UR206-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Aix Marseille Université (AMU), Biologie cellulaire et moléculaire des plantes et des bactéries (BCMPB), Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université de la Méditerranée - Aix-Marseille 2, Université de la Méditerranée - Aix-Marseille 2-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | Applied and Environmental Microbiology Applied and Environmental Microbiology, American Society for Microbiology, 2012, 78 (4), pp.1097-1106. ⟨10.1128/AEM.06522-11⟩ Applied and Environmental Microbiology, 2012, 78 (4), pp.1097-1106. ⟨10.1128/AEM.06522-11⟩ |
| بيانات النشر: | HAL CCSD, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | DNA, Bacterial, Molecular Sequence Data, chemistry.chemical_element, Microbial Sensitivity Tests, 010501 environmental sciences, DNA, Ribosomal, 01 natural sciences, Applied Microbiology and Biotechnology, Bacterial cell structure, 03 medical and health sciences, Bioremediation, Extracellular polymeric substance, Dry weight, RNA, Ribosomal, 16S, Environmental Microbiology, Cluster Analysis, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], Effluent, Phylogeny, ComputingMilieux_MISCELLANEOUS, 0105 earth and related environmental sciences, 2. Zero hunger, 0303 health sciences, Bacteria, Ecology, biology, 030306 microbiology, Spectrum Analysis, Polysaccharides, Bacterial, Biosorption, Sequence Analysis, DNA, biology.organism_classification, Geomicrobiology, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, 6. Clean water, Mercury (element), chemistry, 13. Climate action, Environmental chemistry, Mercuric Chloride, Environmental Pollutants, Food Science, Biotechnology |
| الوصف: | Accumulation of toxic metals in the environment represents a public health and wildlife concern. Bacteria resistant to toxic metals constitute an attractive biomass for the development of systems to decontaminate soils, sediments, or waters. In particular, biosorption of metals within the bacterial cell wall or secreted extracellular polymeric substances (EPS) is an emerging process for the bioremediation of contaminated water. Here the isolation of bacteria from soil, effluents, and river sediments contaminated with toxic metals permitted the selection of seven bacterial isolates tolerant to mercury and associated with a mucoid phenotype indicative of the production of EPS. Inductively coupled plasma-optical emission spectroscopy and transmission electron microscopy in conjunction with X-ray energy dispersive spectrometry revealed that bacteria incubated in the presence of HgCl 2 sequestered mercury extracellularly as spherical or amorphous deposits. Killed bacterial biomass incubated in the presence of HgCl 2 also generated spherical extracellular mercury deposits, with a sequestration capacity (40 to 120 mg mercury per g [dry weight] of biomass) superior to that of live bacteria (1 to 2 mg mercury per g [dry weight] of biomass). The seven strains were shown to produce EPS, which were characterized by Fourier transform-infrared (FT-IR) spectroscopy and chemical analysis of neutral-carbohydrate, uronic acid, and protein contents. The results highlight the high potential of Hg-tolerant bacteria for applications in the bioremediation of mercury through biosorption onto the biomass surface or secreted EPS. |
| اللغة: | English |
| تدمد: | 0099-2240 1098-5336 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::648c350b22f200a287d2988db48970d4 https://hal.archives-ouvertes.fr/hal-02912631 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....648c350b22f200a287d2988db48970d4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00992240 10985336 |
|---|