التفاصيل البيبلوغرافية
| العنوان: |
Dasatinib plus quercetin prevents uterine age-related dysfunction and fibrosis in mice |
| المؤلفون: |
Allancer D. C. Nunes, Michal M. Masternak, Augusto Schneider, James L. Kirkland, Tatiana D. Saccon, Tamara Tchkonia, Marcelo Borges Cavalcante |
| المصدر: |
Aging (Albany NY) |
| بيانات النشر: |
Impact Journals, LLC, 2020. |
| سنة النشر: |
2020 |
| مصطلحات موضوعية: |
Aging, medicine.medical_specialty, mRNA, Dasatinib, Uterus, AKT1, Antioxidants, Mice, 03 medical and health sciences, 0302 clinical medicine, Downregulation and upregulation, Fibrosis, Internal medicine, Gene expression, medicine, Animals, Senolytic, Protein Kinase Inhibitors, PI3K/AKT/mTOR pathway, miRNA, 030304 developmental biology, Uterine Diseases, 0303 health sciences, business.industry, Cell Biology, medicine.disease, 3. Good health, medicine.anatomical_structure, Endocrinology, 030220 oncology & carcinogenesis, senolytics, Drug Therapy, Combination, Female, Quercetin, business, uterine fibrosis, Research Paper, medicine.drug |
| الوصف: |
The uterine fibrosis contributes to gestational outcomes. Collagen deposition in the uterus is related to uterine aging. Senolytic therapies are an option for reducing health complications related to aging. We investigated effects of aging and the senolytic drug combination of dasatinib plus quercetin (D+Q) on uterine fibrosis. Forty mice, 20 young females (03-months) and 20 old females (18-months), were analyzed. Young (Y) and old (O) animals were divided into groups of 10 mice, with one treatment (T) group (YT and OT) and another control © group (YC and OC). Comparative analysis of Pi3k/Akt1/mTor and p53 gene expression and related microRNAs (miR34a, miR34b, miR34c, miR146a, miR449a, miR21a, miR126a, and miR181b) among groups was performed to test effects of age and treatment on collagen deposition pathways. Aging promoted downregulation of the Pi3k/Akt1/mTor signaling pathway (P = 0.005, P = 0.031, and P = 0.028, respectively) as well as a reduction in expression of miR34c (P = 0.029), miR126a (P = 0.009), and miR181b (P = 0.007). D+Q treatment increased p53 gene expression (P = 0.041) and decreased miR34a (P = 0.016). Our results demonstrate a role for the Pi3k/Akt1/mTor signaling pathway in uterine aging and suggest for the first time a possible anti-fibrotic effect in the uterus of D+Q senolytic therapy. |
| تدمد: |
1945-4589 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6507c9ec1aae8808d08f1ab9305918e4
https://doi.org/10.18632/aging.102772 |
| حقوق: |
OPEN |
| رقم الأكسشن: |
edsair.doi.dedup.....6507c9ec1aae8808d08f1ab9305918e4 |
| قاعدة البيانات: |
OpenAIRE |
