Artificial Antigen-Presenting Cell Topology Dictates T Cell Activation
| العنوان: | Artificial Antigen-Presenting Cell Topology Dictates T Cell Activation |
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| المؤلفون: | Annelies C. Wauters, Jari F. Scheerstra, Irma G. Vermeijlen, Roel Hammink, Marjolein Schluck, Laura Woythe, Hanglong Wu, Lorenzo Albertazzi, Carl G. Figdor, Jurjen Tel, Loai K. E. A. Abdelmohsen, Jan C. M. van Hest |
| المساهمون: | Bio-Organic Chemistry, Immunoengineering, Biomedical Engineering, Molecular Biosensing for Med. Diagnostics, ICMS Core, Nanoscopy for Nanomedicine, Institute for Complex Molecular Systems |
| المصدر: | ACS Nano, 16, 6, pp. 15072-15085 ACS Nano, 16, 15072-15085 ACS Nano, 16(9). American Chemical Society |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Technology, Chemistry, Multidisciplinary, Cancer development and immune defence Radboud Institute for Molecular Life Sciences [Radboudumc 2], Materials Science, General Physics and Astronomy, Antigen-Presenting Cells, Materials Science, Multidisciplinary, Lymphocyte Activation, Ligands, Polyethylene Glycols, antibody density, SYNTHETIC DENDRITIC CELLS, All institutes and research themes of the Radboud University Medical Center, DESIGN, nano-immunotherapy, biodegradable polymersomes, General Materials Science, Nanoscience & Nanotechnology, IMMUNOTHERAPY, nanoparticle morphology, Science & Technology, Chemistry, Physical, T cell activation, General Engineering, EXPANSION, Chemistry, artificial antigen-presenting cells, Physical Sciences, Science & Technology - Other Topics, Immunotherapy |
| الوصف: | Nanosized artificial antigen-presenting cells (aAPCs), synthetic immune cell mimics that aim to activate T cells ex or in vivo, offer an effective alternative to cellular immunotherapies. However, comprehensive studies that delineate the effect of nano-aAPC topology, including nanoparticle morphology and ligand density, are lacking. Here, we systematically studied the topological effects of polymersome-based aAPCs on T cell activation. We employed an aAPC library created from biodegradable poly(ethylene glycol)-block-poly(d,l-lactide) (PEG-PDLLA) polymersomes with spherical or tubular shape and variable sizes, which were functionalized with αCD3 and αCD28 antibodies at controlled densities. Our results indicate that high ligand density leads to enhancement in T cell activation, which can be further augmented by employing polymersomes with larger size. At low ligand density, the effect of both polymersome shape and size was more pronounced, showing that large elongated polymersomes better activate T cells compared to their spherical or smaller counterparts. This study demonstrates the capacity of polymersomes as aAPCs and highlights the role of topology for their rational design. ispartof: ACS NANO vol:16 issue:6 pages:15072-15085 ispartof: location:United States status: published |
| وصف الملف: | Print-Electronic; application/pdf |
| تدمد: | 1936-0851 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::652d1fb16074e154aede58d1f05fbaa2 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....652d1fb16074e154aede58d1f05fbaa2 |
| قاعدة البيانات: | OpenAIRE |
Find this issue from the American Chemical Society | تدمد: | 19360851 |
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