Breakfast partly restores the anti-inflammatory function of high-density lipoproteins from patients with type 2 diabetes mellitus
| العنوان: | Breakfast partly restores the anti-inflammatory function of high-density lipoproteins from patients with type 2 diabetes mellitus |
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| المؤلفون: | Willem A. Dik, Monique T. Mulder, Nikita Martens, Roosmarijn F.H. Lemmers, Adrie J.M. Verhoeven, L. C. van Vark-van der Zee, M. van Hoek, Aloysius G Lieverse, Frank P.J. Leijten, Harm R. Haak, Eric J.G. Sijbrands, A.H. Maas, Pieter J. M. Leenen, C.M. Groot-van Ruijven |
| المساهمون: | Internal Medicine, Immunology |
| المصدر: | Atherosclerosis Plus, 44, 43-50. Elsevier Ireland Ltd Atherosclerosis Plus, Vol 44, Iss, Pp 43-50 (2021) |
| بيانات النشر: | Elsevier Ireland Ltd, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | medicine.medical_specialty, medicine.drug_class, High density, Anti-inflammatory, chemistry.chemical_compound, SDG 3 - Good Health and Well-being, Internal medicine, Type 2 diabetes mellitus, Internal Medicine, Medicine, Diseases of the circulatory (Cardiovascular) system, Peg precipitation, Endothelium, Breakfast, VCAM-1, business.industry, Type 2 Diabetes Mellitus, nutritional and metabolic diseases, Retinal, Iodixanol, High-density lipoprotein function, Endocrinology, medicine.anatomical_structure, chemistry, RC666-701, Density gradient ultracentrifugation, lipids (amino acids, peptides, and proteins), Cardiology and Cardiovascular Medicine, business, Density gradient centrifugation, medicine.drug, Artery |
| الوصف: | Background and aims: High-density lipoproteins (HDL) of patients with type 2 diabetes mellitus (T2DM) have impaired anti-inflammatory activities. The anti-inflammatory activity of HDL has been determined ex vivo after isolation by different methods from blood mostly obtained after overnight fasting. We first determined the effect of the HDL isolation method, and subsequently the effect of food intake on the anti-inflammatory function of HDL from T2DM patients. Methods: Blood was collected from healthy controls and T2DM patients after an overnight fast, and from T2DM patients 3 h after breakfast (n = 17 each). HDL was isolated by a two-step density gradient ultracentrifugation in iodixanol (HDLDGUC2), by sequential salt density flotation (HDLSEQ) or by PEG precipitation (HDLPEG). The anti-inflammatory function of HDL was determined by the reduction of the TNFα-induced expression of VCAM-1 in human coronary artery endothelial cells (HCAEC) and retinal endothelial cells (REC). Results: HDL isolated by the three different methods from healthy controls inhibited TNFα-induced VCAM-1 expression in HCAEC. With apoA-I at 0.7 μM, HDLDGUC2 and HDLSEQ were similarly effective (16% versus 14% reduction; n = 3; p > 0.05) but less effective than HDLPEG (28%, p < 0.05). Since ultracentrifugation removes most of the unbound plasma proteins, we used HDLDGUC2 for further experiments. With apoA-I at 3.2 μM, HDL from fasting healthy controls and T2DM patients reduced TNFα-induced VCAM-1 expression in HCAEC by 58 ± 13% and 51 ± 20%, respectively (p = 0.35), and in REC by 42 ± 13% and 25 ± 18%, respectively (p < 0.05). Compared to preprandial HDL, postprandial HDL from T2DM patients reduced VCAM-1 expression by 56 ± 16% (paired test: p < 0.001) in HCAEC and by 34 ± 13% (paired test: p < 0.05) in REC. Conclusions: The ex vivo anti-inflammatory activity of HDL is affected by the HDL isolation method. Two-step ultracentrifugation in an iodixanol gradient is a suitable method for HDL isolation when testing HDL anti-inflammatory function. The anti-inflammatory activity of HDL from overnight fasted T2DM patients is significantly impaired in REC but not in HCAEC. The anti-inflammatory function of HDL is partly restored by food intake. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1878-5050 1567-5688 2667-0909 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::652f463303f7c95371af0dd4af6a5fe5 https://doi.org/10.1016/j.athplu.2021.08.006 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....652f463303f7c95371af0dd4af6a5fe5 |
| قاعدة البيانات: | OpenAIRE |
Full Text via ClinicalKey | تدمد: | 18785050 15675688 26670909 |
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