Phylogenetic and complementation analysis of a single-stranded dna binding protein family from lactococcal phages indicates a non-bacterial origin
| العنوان: | Phylogenetic and complementation analysis of a single-stranded dna binding protein family from lactococcal phages indicates a non-bacterial origin |
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| المؤلفون: | Eric Prestel, Mahendra Mariadassou, Jacek Bardowski, Elena Bidnenko, Agnieszka K. Szczepankowska |
| المساهمون: | MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Department of Microbial Biochemistry, Institute of Biochemistry and Biophysics PAS, Unité Mathématique, Informatique et Génome (MIG), Institut National de la Recherche Agronomique (INRA), Bidnenko, Elena |
| المصدر: | PLoS ONE PLoS ONE, Public Library of Science, 2011, 6 (11), ⟨10.1371/journal.pone.0026942⟩ Plos One 11 (6), . (2011) PLoS ONE, Vol 6, Iss 11, p e26942 (2011) |
| بيانات النشر: | HAL CCSD, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | [SDV]Life Sciences [q-bio], Gene Identification and Analysis, lcsh:Medicine, medicine.disease_cause, Biochemistry, Genome, gene-transfer, Lactococcus, Cluster Analysis, Bacteriophages, lcsh:Science, analyse phylogénique, Phylogeny, bactérie, Genetics, 0303 health sciences, Multidisciplinary, Archaeal Evolution, escherichia-coli, identification, famille de protéine, Gene Classes, Research Article, Gene Flow, Archaeans, Protein family, DNA, Single-Stranded, Sequence alignment, Biology, Microbiology, DNA-binding protein, Viral Evolution, Molecular Genetics, 03 medical and health sciences, stomatognathic system, Virology, DNA-binding proteins, Evolutionary Modeling, Escherichia coli, medicine, Protein classes, Gene, 030304 developmental biology, Bacterial Evolution, 030306 microbiology, lcsh:R, Genetic Complementation Test, DNA replication, Proteins, Computational Biology, Bacteriology, Protein superfamily, stomatognathic diseases, lcsh:Q, Population Genetics |
| الوصف: | BACKGROUND: The single-stranded-nucleic acid binding (SSB) protein superfamily includes proteins encoded by different organisms from Bacteria and their phages to Eukaryotes. SSB proteins share common structural characteristics and have been suggested to descend from an ancestor polypeptide. However, as other proteins involved in DNA replication, bacterial SSB proteins are clearly different from those found in Archaea and Eukaryotes. It was proposed that the corresponding genes in the phage genomes were transferred from the bacterial hosts. Recently new SSB proteins encoded by the virulent lactococcal bacteriophages (Orf14(bIL67)-like proteins) have been identified and characterized structurally and biochemically. METHODOLOGY/PRINCIPAL FINDINGS: This study focused on the determination of phylogenetic relationships between Orf14(bIL67)-like proteins and other SSBs. We have performed a large scale phylogenetic analysis and pairwise sequence comparisons of SSB proteins from different phyla. The results show that, in remarkable contrast to other phage SSBs, the Orf14(bIL67)-like proteins form a distinct, self-contained and well supported phylogenetic group connected to the archaeal SSBs. Functional studies demonstrated that, despite the structural and amino acid sequence differences from bacterial SSBs, Orf14(bIL67) protein complements the conditional lethal ssb-1 mutation of Escherichia coli. CONCLUSIONS/SIGNIFICANCE: Here we identified for the first time a group of phages encoded SSBs which are clearly distinct from their bacterial counterparts. All methods supported the recognition of these phage proteins as a new family within the SSB superfamily. Our findings suggest that unlike other phages, the virulent lactococcal phages carry ssb genes that were not acquired from their hosts, but transferred from an archaeal genome. This represents a unique example of a horizontal gene transfer between Archaea and bacterial phages. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::65f4b06107769390f2a3e93459f02de9 https://hal.archives-ouvertes.fr/hal-01191212 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....65f4b06107769390f2a3e93459f02de9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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