Additional file 1: Table S1. Cultural characteristics of Streptomyces spectabilis KCTC9218T on different growth media. Table S2. List of genes used for autoMLST analysis. Fig. S1. Location of the sampling sites in South Korea. Red marks indicate areas where soil samples were obtained. Fig. S2. Morphological characteristics of Streptomyces spectabilis KCTC9218T grown on Bennett’s agar media. Fig. S3. LC-MS analysis of (A) spectinabilin extracted from the cell pellets and media broth of S. spectabilis KCTC9218T and Streptomyces sp. AN091965 cultures and (B) their MS/MS fragmentation analysis. Fig. S4. LC-MS chromatogram of metabolite fractions collected during purification of spectinabilin from the crude extract of Streptomyces sp. AN091965 culture broth and cell pellets. Spectinabilin was eluted from silica column at 100 % methanol. Fig. S5. LC-MS spectrum of spectinabilin (yellow), undecylprodigiosin (orange), and streptorubin B (green) from S. spectabilis KCTC9218T and Streptomyces sp. AN091965 cultures.