The Mia/Cd-rap gene expression is downregulated by the high-mobility group A proteins in mouse pituitary adenomas
| العنوان: | The Mia/Cd-rap gene expression is downregulated by the high-mobility group A proteins in mouse pituitary adenomas |
|---|---|
| المؤلفون: | Dario Palmieri, Monica Fedele, Paolo Chieffi, Mogens Kruhøffer, Floriana Forzati, Gaetano Lombardi, Rosa Visone, Paolo Cappabianca, Antonio Barbieri, Alfredo Fusco, Ivana De Martino |
| المساهمون: | DE MARTINO, I, Visone, R, Palmieri, D, Cappabianca, P, Chieffi, Paolo, Forzati, F, Barbieri, A, Kruhoffer, M, Lombardi, G, Fusco, A, Fedele, M., DE MARTINO, Ivana, Visone, R., Palmieri, Dario, Cappabianca, Paolo, Chieffi, P., Forzati, F., Barbieri, A., Kruhoffer, M., Lombardi, Gaetano, Fusco, Alfredo |
| المصدر: | Endocrine-related cancer (2007). info:cnr-pdr/source/autori:De Martino I, Visone R, Palmieri D, Cappabianca P, Chieffi P, Forzati F, Barbieri A, Kruhoffer M, Lombardi G, Fusco A, Fedele M/titolo:The Mia%2FCd-rap gene expression is downregulated by the high-mobility group A proteins in mouse pituitary adenomas./doi:/rivista:Endocrine-related cancer/anno:2007/pagina_da:/pagina_a:/intervallo_pagine:/volume |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | Adenoma, Cancer Research, Endocrinology, Diabetes and Metabolism, Down-Regulation, pituitary adenoma, Mice, Endocrinology, HMGA2, Mia/Cd rap, Gene expression, Tumor Cells, Cultured, Transcriptional regulation, Animals, Cluster Analysis, E2F1, Pituitary Neoplasms, HMGA, Promoter Regions, Genetic, HMGA Proteins, Gene, Cell Proliferation, Oligonucleotide Array Sequence Analysis, Extracellular Matrix Proteins, biology, Gene Expression Profiling, Wild type, Molecular biology, Rats, Gene Expression Regulation, Neoplastic, Oncology, biology.protein |
| الوصف: | The high-mobility group A (HMGA) family of proteins orchestrates the assembly of nucleoprotein structures playing important roles in gene transcription, recombination, and chromatin structure through a complex network of protein–DNA and protein–protein interactions. Recently, we have generated transgenic mice carrying wild type or truncated HMGA2 genes under the transcriptional control of the cytomegalovirus promoter. These mice developed pituitary adenomas secreting prolactin and GH mainly due to an increased E2F1 activity, directly consequent to the HMGA2 overexpression. To identify other genes involved in the process of pituitary tumorigenesis induced by the HMGA2 gene, in this study we have analyzed the gene expression profile of three HMGA2-pituitary adenomas in comparison with a pool of ten normal pituitary glands from control mice, using the Affymetrix MG MU11K oligonucleotide array representing ~13 000 unique genes. We have identified 82 transcripts that increased and 72 transcripts that decreased at least four-fold in all the mice pituitary adenomas analyzed compared with normal pituitary glands. Among these genes, we focused our attention on the Mia/Cd-rap gene, whose expression was essentially suppressed in all of the pituitary adenomas tested by the microarray. We demonstrated that the HMGA proteins directly bind to the promoter of the Mia/Cd-rap gene and are able to downregulate its expression. In order to understand a possible role of Mia/Cd-rap in pituitary cell growth, we performed a colony assay in GH3 and GH4 cells. Interestingly, Mia/Cd-rap expression inhibits their proliferation, suggesting a potential tumor suppressor role of Mia/Cd-rap in pituitary cells. |
| وصف الملف: | STAMPA |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::65fd46fbf563bd6caac9abdba1e70616 http://hdl.handle.net/11591/187479 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....65fd46fbf563bd6caac9abdba1e70616 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |