Identification of Polyethylene Glycol-Resistant Macrophages on Stealth Imaging in Vitro Using Fluorescent Organosilica Nanoparticles
| العنوان: | Identification of Polyethylene Glycol-Resistant Macrophages on Stealth Imaging in Vitro Using Fluorescent Organosilica Nanoparticles |
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| المؤلفون: | Kazue Miyamoto, Kazuki Horikawa, Koichiro Hayashi, Michihiro Nakamura, Toshinari Kori, Takafumi Kanadani, Mutsuki Nakano |
| المصدر: | ACS Nano. 9:1058-1071 |
| بيانات النشر: | American Chemical Society (ACS), 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Materials science, Surface Properties, Kinetics, General Physics and Astronomy, Nanoparticle, Nanotechnology, Polyethylene glycol, Polyethylene Glycols, Flow cytometry, Mice, chemistry.chemical_compound, Single-cell analysis, PEG ratio, medicine, Animals, Organosilicon Compounds, General Materials Science, Fluorescent Dyes, Drug Carriers, medicine.diagnostic_test, Macrophages, General Engineering, Biological Transport, Silanes, Fluorescence, In vitro, Microscopy, Fluorescence, chemistry, Biophysics, Nanoparticles, Biomarkers |
| الوصف: | An in vitro imaging system to evaluate the stealth function of nanoparticles against mouse macrophages was established using fluorescent organosilica nanoparticles. Surface-functionalized organosilica nanoparticles with polyethylene glycol (PEG) were prepared by a one-step process, resulting in a brush-type PEG layer. A simultaneous dual-particle administration approach enabled us to evaluate the stealth function of nanoparticles with respect to single cells using time-lapse fluorescent microscopic imaging and flow cytometry analyses. Single-cell imaging and analysis revealed various patterns and kinetics of bare and PEGylated nanoparticle uptake. The PEGylated nanoparticles revealed a stealth function against most macrophages (PEG-sensitive macrophages); however, a stealth function against certain macrophages (PEG-insensitive macrophages) was not observed. We identified and characterized the PEG-resistant macrophages that could take up PEGylated nanoparticles at the same level as bare nanoparticles. |
| تدمد: | 1936-086X 1936-0851 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::660ab01a060f5108effed1e495f93558 https://doi.org/10.1021/nn502319r |
| رقم الأكسشن: | edsair.doi.dedup.....660ab01a060f5108effed1e495f93558 |
| قاعدة البيانات: | OpenAIRE |
Find this issue from the American Chemical Society | تدمد: | 1936086X 19360851 |
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