Eukaryotic translation elongation factor 1-alpha 1 inhibits p53 and p73 dependent apoptosis and chemotherapy sensitivity
| العنوان: | Eukaryotic translation elongation factor 1-alpha 1 inhibits p53 and p73 dependent apoptosis and chemotherapy sensitivity |
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| المؤلفون: | Fiona Robinson, Alvaro Blanch, Ian R. Watson, Meredith S. Irwin, Lynn S. Cheng |
| المصدر: | PLoS ONE, Vol 8, Iss 6, p e66436 (2013) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Small interfering RNA, Cancer Treatment, Regulator, Gene Expression, lcsh:Medicine, Apoptosis, Biochemistry, Peptide Elongation Factor 1, Molecular cell biology, RNA interference, Basic Cancer Research, RNA, Small Interfering, lcsh:Science, Etoposide, Cellular Stress Responses, Multidisciplinary, Cell Death, Nuclear Proteins, Transfection, Nucleic acids, Oncology, Gene Knockdown Techniques, Medicine, Research Article, Antineoplastic Agents, Biology, Eukaryotic translation, DNA-binding proteins, Humans, Gene silencing, Transcription factor, Tumor Suppressor Proteins, lcsh:R, Proteins, Protein interactions, Tumor Protein p73, Regulatory proteins, Chemotherapy and Drug Treatment, Eukaryotic translation elongation factor 1 alpha 1, Elongation factor, HEK293 Cells, Doxorubicin, Drug Resistance, Neoplasm, Cancer research, RNA, Camptothecin, lcsh:Q, Cisplatin, Tumor Suppressor Protein p53, HeLa Cells |
| الوصف: | The p53 family of transcription factors is a key regulator of cell proliferation and death. In this report we identify the eukaryotic translation elongation factor 1-alpha 1 (eEF1A1) to be a novel p53 and p73 interacting protein. Previous studies have demonstrated that eEF1A1 has translation-independent roles in cancer. We report that overexpression of eEF1A1 specifically inhibits p53-, p73- and chemotherapy-induced apoptosis resulting in chemoresistance. Short-interfering RNA-mediated silencing of eEF1A1 increases chemosensitivity in cell lines bearing wild type p53, but not in p53 null cells. Furthermore, silencing of eEF1A1 partially rescues the chemoresistance observed in response to p53 or p73 knockdown, suggesting that eEF1A1 is a negative regulator of the pro-apoptotic function of p53 and p73. Thus, in the context of p53-family signaling, eEF1A1 has anti-apoptotic properties. These findings identify a novel mechanism of regulation of the p53 family of proteins by eEF1A1 providing additional insight into potential targets to sensitize tumors to chemotherapy. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6627abb4591b66237f6de881dda42a08 http://europepmc.org/articles/PMC3682968?pdf=render |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....6627abb4591b66237f6de881dda42a08 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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