High-Throughput Continuous-Flow Separation in a Micro Free-Flow Electrophoresis Glass Chip Based on Laser Microfabrication
| العنوان: | High-Throughput Continuous-Flow Separation in a Micro Free-Flow Electrophoresis Glass Chip Based on Laser Microfabrication |
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| المؤلفون: | Aodong Zhang, Jian Xu, Xiaolong Li, Zijie Lin, Yunpeng Song, Xin Li, Zhenhua Wang, Ya Cheng |
| المصدر: | Sensors; Volume 22; Issue 3; Pages: 1124 Sensors, Vol 22, Iss 1124, p 1124 (2022) |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Electrophoresis, Chemical technology, Lasers, TP1-1185, laser-assisted chemical etching, Biochemistry, Atomic and Molecular Physics, and Optics, Analytical Chemistry, femtosecond laser microfabrication, glass microfluidics, micro free-flow electrophoresis, continuous-flow separation, Microtechnology, Fluorescein, Glass, Electrical and Electronic Engineering, Instrumentation |
| الوصف: | Micro free-flow electrophoresis (μFFE) provides a rapid and straightforward route for the high-performance online separation and purification of targeted liquid samples in a mild manner. However, the facile fabrication of a μFFE device with high throughput and high stability remains a challenge due to the technical barriers of electrode integration and structural design for the removal of bubbles for conventional methods. To address this, the design and fabrication of a high-throughput μFFE chip are proposed using laser-assisted chemical etching of glass followed by electrode integration and subsequent low-temperature bonding. The careful design of the height ratio of the separation chamber and electrode channels combined with a high flow rate of buffer solution allows the efficient removal of electrolysis-generated bubbles along the deep electrode channels during continuous-flow separation. The introduction of microchannel arrays further enhances the stability of on-chip high-throughput separation. As a proof-of-concept, high-performance purification of fluorescein sodium solution with a separation purity of ~97.9% at a voltage of 250 V from the mixture sample solution of fluorescein sodium and rhodamine 6G solution is demonstrated. |
| وصف الملف: | application/pdf |
| تدمد: | 1424-8220 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::664b4654cffde51c883b523b7f54f03e https://pubmed.ncbi.nlm.nih.gov/35161869 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....664b4654cffde51c883b523b7f54f03e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14248220 |
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