Interleukin 6 Plays a Role in the Migration of Magnetically Levitated Mesenchymal Stem Cells Spheroids
| العنوان: | Interleukin 6 Plays a Role in the Migration of Magnetically Levitated Mesenchymal Stem Cells Spheroids |
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| المؤلفون: | Matthew J. Dalby, Carol-Anne Smith, Catherine C. Berry, Jake Casson, Sam O’Kane |
| المصدر: | Applied Sciences, Vol 8, Iss 3, p 412 (2018) Applied Sciences; Volume 8; Issue 3; Pages: 412 |
| بيانات النشر: | MDPI AG, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, magnetic nanoparticles, medicine.medical_treatment, Cell, Matrix metalloproteinase, lcsh:Technology, lcsh:Chemistry, 03 medical and health sciences, 0302 clinical medicine, medicine, General Materials Science, Instrumentation, lcsh:QH301-705.5, mesenchymal stem cell, Fluid Flow and Transfer Processes, Chemistry, lcsh:T, Process Chemistry and Technology, Mesenchymal stem cell, General Engineering, Spheroid, Chemotaxis, type I collagen, lcsh:QC1-999, Computer Science Applications, Cell biology, matrix metalloprotease, 030104 developmental biology, medicine.anatomical_structure, Cytokine, lcsh:Biology (General), lcsh:QD1-999, spheroid, lcsh:TA1-2040, 030220 oncology & carcinogenesis, Bone marrow, lcsh:Engineering (General). Civil engineering (General), tissue inhibitor of metalloprotease, lcsh:Physics, Homing (hematopoietic) |
| الوصف: | Mesenchymal stem cells (MSCs) reside quiescently within a specialised ‘niche’ environment in the bone marrow. However, following appropriate signalling cues, MSCs mobilise and migrate out from the niche, typically toward either sites of injury (a regenerative response) or toward primary tumours (an intrinsic homing response, which promotes MSCs as cellular vectors for therapeutic delivery). To date, very little is known about MSC mobilisation. By adopting a 3D MSC niche model, whereby MSC spheroids are cultured within a type I collagen gel, recent studies have highlighted interleukin-6 (IL-6) as a key cytokine involved in MSC migration. Herein, the ability of IL-6 to induce MSC migration was further investigated, and the key matrix metalloproteinases used to effect cell mobilisation were identified. Briefly, the impact of IL-6 on the MSC migration in a two-dimensional model systems was characterised—both visually using an Ibidi chemotaxis plate array (assessing for directional migration) and then via a standard 2D monolayer experiment, where cultured cells were challenged with IL-6 and extracted media tested using an Abcam Human MMP membrane antibody array. The 2D assay displayed a strong migratory response toward IL-6 and analysis of the membrane arrays data showed significant increases of several key MMPs. Both data sets indicated that IL-6 is important in MSC mobilisation and migration. We also investigated the impact of IL-6 induction on MSCs in 3D spheroid culture, serving as a simplistic model of the bone marrow niche, characterised by fluorescently tagged magnetic nanoparticles and identical membrane antibody arrays. An increase in MMP levels secreted by cells treated with 1 ng/mL IL-6 versus control conditions was noted in addition to migration of cells away from the central spheroid mass. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2076-3417 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::667a11e874972c1644f5468c2be08580 http://www.mdpi.com/2076-3417/8/3/412 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....667a11e874972c1644f5468c2be08580 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20763417 |
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