Application of poxvirus K3 ortholog as a positive selection marker for constructing recombinant vaccinia viruses with modified host range
| العنوان: | Application of poxvirus K3 ortholog as a positive selection marker for constructing recombinant vaccinia viruses with modified host range |
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| المؤلفون: | Yvon Deschambault, Jingxin Cao, Jessie Varga, Christine Layne |
| المصدر: | MethodsX, Vol 7, Iss, Pp 100918-(2020) MethodsX |
| بيانات النشر: | Elsevier, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | RNase P, viruses, Clinical Biochemistry, 010501 environmental sciences, Biology, Recombinant virus, 01 natural sciences, Virus, law.invention, 03 medical and health sciences, chemistry.chemical_compound, law, Vaccinia, Vector (molecular biology), lcsh:Science, Gene, k3L, 030304 developmental biology, 0105 earth and related environmental sciences, Immunology and Microbiology, 0303 health sciences, Transfection, Virology, Recombination, Medical Laboratory Technology, chemistry, Poxvirus, Host range, Recombinant DNA, lcsh:Q |
| الوصف: | Vaccinia virus is capable of replicating in a wide range of vertebrate animal cells. However, deletion of the two virus host range genes, E3L and K3L, would render replication of the virus abortive in all the cell types tested, except the cells with defective PKR and RNase L activity. By expressing different poxvirus K3 ortholog proteins in the E3L and K3L double knockout vaccinia virus, we can construct a mutant vaccinia virus with modified host range. Here, using poxvirus K3 ortholog as a positive selection marker, we developed a proof-of-concept protocol to construct recombinant vaccinia viruses expressing foreign proteins. Such a recombinant virus has a modified host range in comparison to wild-type vaccinia virus. This protocol offers the following advantages:➢Cheap: no additional selection reagent is required.➢Highly efficient: nearly all recombinant virus plaques picked would be the stable recombinant virus expressing the protein of interest.➢Rapid: starting from transfection with the recombinant DNA vector, a purified recombinant virus expressing the protein of interest could be obtained within a week. Graphical abstract Image, graphical abstract |
| اللغة: | English |
| تدمد: | 2215-0161 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::66cc42b88306dadea9ff8852dd2b8b50 http://www.sciencedirect.com/science/article/pii/S2215016120301370 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....66cc42b88306dadea9ff8852dd2b8b50 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22150161 |
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