Differences in Vascular Bed Disease Susceptibility Reflect Differences in Gene Expression Response to Atherogenic Stimuli
| العنوان: | Differences in Vascular Bed Disease Susceptibility Reflect Differences in Gene Expression Response to Atherogenic Stimuli |
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| المؤلفون: | Ivette Estay, Amir Ben-Dor, Ramendra K. Kundu, Zohar Yakhini, Raymond Tabibiazar, David Deng, Robert Kincaid, Aditya Vailaya, Laurakay Bruhn, Anya Tsalenko, Thomas Quertermous |
| المصدر: | Circulation Research. 98:200-208 |
| بيانات النشر: | Ovid Technologies (Wolters Kluwer Health), 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | Cell type, Endothelium, Physiology, Biology, beta-Crystallin A Chain, Gene expression, medicine, Humans, Saphenous Vein, Gene, Cells, Cultured, Cell Proliferation, Oligonucleotide Array Sequence Analysis, Tumor Necrosis Factor-alpha, Vascular disease, Gene Expression Profiling, Endothelial Cells, Atherosclerosis, medicine.disease, Coronary Vessels, Immunohistochemistry, Lipoproteins, LDL, Gene expression profiling, medicine.anatomical_structure, Immunology, Cancer research, Tumor necrosis factor alpha, Disease Susceptibility, Cardiology and Cardiovascular Medicine, Immunostaining, Interleukin-1 |
| الوصف: | Atherosclerosis occurs predominantly in arteries and only rarely in veins. The goal of this study was to test whether differences in the molecular responses of venous and arterial endothelial cells (ECs) to atherosclerotic stimuli might contribute to vascular bed differences in susceptibility to atherosclerosis. We compared gene expression profiles of primary cultured ECs from human saphenous vein (SVEC) and coronary artery (CAEC) exposed to atherogenic stimuli. In addition to identifying differentially expressed genes, we applied statistical analysis of gene ontology and pathway annotation terms to identify signaling differences related to cell type and stimulus. Differential gene expression of untreated venous and arterial endothelial cells yielded 285 genes more highly expressed in untreated SVEC ( P 1.5). These genes represented various atherosclerosis-related pathways including responses to proliferation, oxidoreductase activity, antiinflammatory responses, cell growth, and hemostasis functions. Moreover, stimulation with oxidized LDL induced dramatically greater gene expression responses in CAEC compared with SVEC, relating to adhesion, proliferation, and apoptosis pathways. In contrast, interleukin 1β and tumor necrosis factor α activated similar gene expression responses in both CAEC and SVEC. The differences in functional response and gene expression were further validated by an in vitro proliferation assay and in vivo immunostaining of αβ-crystallin protein. Our results strongly suggest that different inherent gene expression programs in arterial versus venous endothelial cells contribute to differences in atherosclerotic disease susceptibility. |
| تدمد: | 1524-4571 0009-7330 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::66d1961e7003cd87c03bc227d2d85755 https://doi.org/10.1161/01.res.0000200738.50997.f2 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....66d1961e7003cd87c03bc227d2d85755 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15244571 00097330 |
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