Developmental expression of the cucumber Cs-XTH1 and Cs-XTH3 genes, encoding xyloglucan endotransglucosylase/hydrolases, can be influenced by mechanical stimuli
| العنوان: | Developmental expression of the cucumber Cs-XTH1 and Cs-XTH3 genes, encoding xyloglucan endotransglucosylase/hydrolases, can be influenced by mechanical stimuli |
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| المؤلفون: | Marcin Filipecki, Robert Malinowski, Michał M. Godlewski, Sabina Zuzga, Stefan Malepszy, Anita Wiśniewska, Stephen C. Fry, Andrzej K. Noyszewski, Anna Barczak-Brzyżek |
| المصدر: | Malinowski, R, Fry, S C, Zuzga, S, Wiśniewska, A, Godlewski, M, Noyszewski, A, Barczak‑Brzyżek, A, Malepszy, S & Filipecki, M 2018, ' Developmental expression of the cucumber Cs-XTH1 and Cs-XTH3 genes, encoding xyloglucan endotransglucosylase/hydrolases, can be influenced by mechanical stimuli ', Acta Physiologiae Plantarum, vol. 40, pp. 130 . https://doi.org/10.1007/s11738-018-2707-7 |
| بيانات النشر: | Springer Science and Business Media LLC, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Somatic embryogenesis, Physiology, Cell, Developmental and adaptive gene regulation, Plant Science, Biology, 01 natural sciences, 03 medical and health sciences, medicine, Arabidopsis thaliana, Xyloglucan endotransglucosylase/hydrolase (XTH), Gene, Cucumber, Xyloglucan–oligosaccharide–sulphorhodamine conjugate (XGO-SR), Plant physiology, Xyloglucan endotransglucosylase, biology.organism_classification, Cell biology, 030104 developmental biology, medicine.anatomical_structure, Regulatory sequence, Cucumis sativus, Agronomy and Crop Science, Cucumis, 010606 plant biology & botany |
| الوصف: | The expression of two genes encoding xyloglucan endotransglucosylase/hydrolases (XTHs), Cs-XTH1 and Cs-XTH3, was upregulated during the onset of cucumber somatic embryogenesis. As a means of characterising the developmental regulation of these genes, the activity of the respective upstream regulatory regions was investigated in seedlings and somatic embryos of Arabidopsis thaliana and Cucumis sativus. GUS assays revealed that both genes are under developmental control. In addition, elevated promoter activity was found in the tension-bearing regions of the plant and in response to touch and wounding, which is consistent with the existence of numerous stress-related cis elements in the 5′-regulatory regions. In vivo xyloglucan endotransglucosylase (XET) action assays were performed to gain an overview on the role of XTHs during somatic embryogenesis. The highest XET action was observed in the external cell layers of somatic embryos in the cotyledonary region and in the presumptive region of peg formation. Based on the results, we propose a dual mechanism (one developmental and the second adaptive) for the regulation of Cs-XTH1 and Cs-XTH3 activity wherein the developmental pattern can be modified by mechanical stimuli. |
| وصف الملف: | application/pdf |
| تدمد: | 1861-1664 0137-5881 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6705daf510d9d95fe70cc403dcebe72a https://doi.org/10.1007/s11738-018-2707-7 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....6705daf510d9d95fe70cc403dcebe72a |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 18611664 01375881 |
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