A genetic screen for temperature-sensitive morphogenesis-defective Caenorhabditis elegans mutants
| العنوان: | A genetic screen for temperature-sensitive morphogenesis-defective Caenorhabditis elegans mutants |
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| المؤلفون: | Zhirong Bao, Josh Lowry, Erin Clifford, Francesca Fennell, Nhah Nguyen Tran, Thalia Padilla, Martha Avila-Zavala, Yuqi Yang, Hong Shao, Danielle R Hamill, Austin M. Harvey, Bruce Bowerman, Molly Christine Jud, Alexander K Miller |
| المصدر: | G3: Genes, Genomes, Genetics, Vol 11, Iss 4 (2021) G3: Genes|Genomes|Genetics |
| بيانات النشر: | Oxford University Press, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | AcademicSubjects/SCI01140, collagen, AcademicSubjects/SCI00010, extracellular matrix, Mutant, Morphogenesis, QH426-470, AcademicSubjects/SCI01180, glycosyltransferase, 03 medical and health sciences, 0302 clinical medicine, Live cell imaging, Embryonic morphogenesis, Genetics, Animals, Cell adhesion, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Molecular Biology, Genetics (clinical), 030304 developmental biology, 0303 health sciences, biology, epidermal cells, Mutant Screen Report, Temperature, biology.organism_classification, Cell biology, Multicellular organism, C. elegans embryonic morphogenesis, AcademicSubjects/SCI00960, Epidermis, 030217 neurology & neurosurgery, Genetic screen |
| الوصف: | Morphogenesis involves coordinated cell migrations and cell shape changes that generate tissues and organs, and organize the body plan. Cell adhesion and the cytoskeleton are important for executing morphogenesis, but their regulation remains poorly understood. As genes required for embryonic morphogenesis may have earlier roles in development, temperature-sensitive embryonic-lethal mutations are useful tools for investigating this process. From a collection of ∼200 such Caenorhabditis elegans mutants, we have identified 17 that have highly penetrant embryonic morphogenesis defects after upshifts from the permissive to the restrictive temperature, just prior to the cell shape changes that mediate elongation of the ovoid embryo into a vermiform larva. Using whole genome sequencing, we identified the causal mutations in seven affected genes. These include three genes that have roles in producing the extracellular matrix, which is known to affect the morphogenesis of epithelial tissues in multicellular organisms: the rib-1 and rib-2 genes encode glycosyltransferases, and the emb-9 gene encodes a collagen subunit. We also used live imaging to characterize epidermal cell shape dynamics in one mutant, or1219ts, and observed cell elongation defects during dorsal intercalation and ventral enclosure that may be responsible for the body elongation defects. These results indicate that our screen has identified factors that influence morphogenesis and provides a platform for advancing our understanding of this fundamental biological process. |
| اللغة: | English |
| تدمد: | 2160-1836 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::674a3d00507a5fd72f6991876ba27ebb https://academic.oup.com/g3journal/article-lookup/doi/10.1093/g3journal/jkab026 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....674a3d00507a5fd72f6991876ba27ebb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 21601836 |
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