Visualizing the innate and adaptive immune responses underlying allograft rejection by two-photon microscopy
| العنوان: | Visualizing the innate and adaptive immune responses underlying allograft rejection by two-photon microscopy |
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| المؤلفون: | Philippe Bousso, Susanna Celli, Matthew L. Albert |
| المساهمون: | Dynamiques des Réponses Immunes, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Immunobiologie des Cellules Dendritiques, This work was supported by INSERM, Institut Pasteur and a Marie Curie Excellence grant, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| المصدر: | Nature Medicine Nature Medicine, Nature Publishing Group, 2011, 17 (6), pp.744-9. ⟨10.1038/nm.2376⟩ Nature Medicine, 2011, 17 (6), pp.744-9. ⟨10.1038/nm.2376⟩ |
| بيانات النشر: | Springer Science and Business Media LLC, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Graft Rejection, MESH: Langerhans Cells/ultrastructure, Pathology, medicine.medical_specialty, MESH: Adaptive Immunity/immunology, Adaptive Immunity, CD8-Positive T-Lymphocytes, Biology, General Biochemistry, Genetics and Molecular Biology, Mice, MESH: Skin/immunology, MESH: Skin/ultrastructure, MESH: Skin Transplantation/immunology, 03 medical and health sciences, 0302 clinical medicine, Immune system, Two-photon excitation microscopy, Antigen, MESH: Mice, Inbred C57BL, MESH: Microscopy, Fluorescence, Multiphoton/methods, medicine, Animals, MESH: Animals, MESH: Mice, Lymph node, Skin, 030304 developmental biology, 0303 health sciences, Graft rejection, MESH: Langerhans Cells/immunology, Skin Transplantation, General Medicine, Intravital Imaging, Immunity, Innate, Mice, Inbred C57BL, MESH: Graft Rejection/immunology, Microscopy, Fluorescence, Multiphoton, surgical procedures, operative, medicine.anatomical_structure, MESH: CD8-Positive T-Lymphocytes/immunology, Allograft rejection, Langerhans Cells, Immunology, [SDV.IMM]Life Sciences [q-bio]/Immunology, MESH: CD8-Positive T-Lymphocytes/ultrastructure, MESH: Immunity, Innate, 030215 immunology |
| الوصف: | International audience; Transplant rejection involves a coordinated attack of the innate and the adaptive immune systems of the host. To investigate this dynamic process and the contributions of both donor and host cells, we developed an ear skin graft model suitable for intravital imaging. We found that donor dermal dendritic cells (DCs) migrated rapidly from the graft and were replaced by host CD11b(+) mononuclear cells. The infiltrating host cells captured donor antigen, reached the draining lymph node and cross-primed graft-reactive CD8(+) T cells. Furthermore, we defined the mechanisms by which host T cells target graft cells. We found that primed T cells entered the graft from the surrounding tissue and localized selectively at the dermis-epidermis junction. Later, CD8(+) T cells disseminated throughout the graft and many became arrested. These results provide insights into the antigen presentation pathway and the stepwise progression of CD8(+) T cell activity, thereby offering a framework for evaluating how immunotherapy might abrogate the key steps in allograft rejection. |
| تدمد: | 1546-170X 1078-8956 1744-7933 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6780d90841207590aa7df0ac7901cef3 https://doi.org/10.1038/nm.2376 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....6780d90841207590aa7df0ac7901cef3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1546170X 10788956 17447933 |
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