Euphresco Sendo: An international laboratory comparison study of molecular tests for
| العنوان: | Euphresco Sendo: An international laboratory comparison study of molecular tests for |
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| المؤلفون: | Toos Dekker, Marga P. E. van Gent-Pelzer, Margriet Boerma, Ilona Kibildiene, Kerstin Flath, Anatolii Karelov, Olga Afanasenko, Ian P. Adams, Bart T. L. H. van de Vossenberg, Ani Besheva, Kurt Heungens, Marcel Westenberg, James Choiseul, Jarosław Przetakiewicz, Gerard van Leeuwen, Alexandra Schlenzig, Vera Yakovleva |
| المصدر: | European Journal of Plant Pathology, 151(3), 757-766 European Journal of Plant Pathology 151 (2018) 3 European Journal of Plant Pathology |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Veterinary medicine, Synchytrium endobioticum, Routine testing, Test Performance Study, Diagnostic Specificity, Plant Science, Horticulture, EPPO diagnostic standard, 01 natural sciences, Article, 03 medical and health sciences, Biointeractions and Plant Health, Validation, Resting spore, biology, Inter-laboratory comparison study, fungi, Test performance study inter-laboratory comparison study, biology.organism_classification, 030104 developmental biology, Potato wart disease, Comparison study, Test performance, Agronomy and Crop Science, 010606 plant biology & botany |
| الوصف: | An international test performance study (TPS) was organised to generate validation data for three molecular Synchytrium endobioticum tests: van den Boogert et al. (European Journal of Plant Pathology 113, 47–57, 2005), and van Gent-Pelzer et al. (European Journal of Plant Pathology, 126, 129-133, 2010) for the detection of S. endobioticum, and the pathotype 1(D1) identification test described by Bonants et al. (European Journal of Plant Pathology, 143, 495-506, 2015). Two TPS rounds were organised focussing on different test matrices, i.e. round 1: warted potato tissue, and round 2: resting spore suspensions. When using the tests for detection and identification of S. endobioticum in warted potato tissue, no significant differences were observed for diagnostic sensitivity, diagnostic specificity, overall accuracy, analytical sensitivity and robustness. When using the tests for detection and identification of S. endobioticum in resting spore suspensions, the van den Boogert and van Gent-Pelzer tests significantly outperform the Bonants test for diagnostic sensitivity and diagnostic specificity. For overall accuracy and analytical sensitivity, the van Gent-Pelzer significantly outperforms the van den Boogert and Bonants tests and is regarded as the test of choice when identifying S. endobioticum from resting spores. Tests regarded fit for purpose for routine testing of wart material and resting spore suspensions are proposed for the update of EPPO standard PM7/28(1) Synchytrium endobioticum. Electronic supplementary material The online version of this article (10.1007/s10658-017-1411-6) contains supplementary material, which is available to authorized users. |
| وصف الملف: | application/pdf |
| تدمد: | 0929-1873 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::67aeef1d6c74ee3f27222cf3b53d82a9 https://pubmed.ncbi.nlm.nih.gov/31007393 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....67aeef1d6c74ee3f27222cf3b53d82a9 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 09291873 |
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