Application of MALDI-TOF MS to rapid identification of anaerobic bacteria
| العنوان: | Application of MALDI-TOF MS to rapid identification of anaerobic bacteria |
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| المؤلفون: | Bing Gu, Mingzhu Shan, Xuhua Mao, Ying Chen, Ying Li, Qiuju Zhu, Zuobin Zhu, Mingju Yan, Hongchun Li |
| المصدر: | BMC Infectious Diseases, Vol 19, Iss 1, Pp 1-11 (2019) BMC Infectious Diseases |
| بيانات النشر: | BMC, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Propionibacterium, 030106 microbiology, Prevotella, Veillonella, Microbiology, lcsh:Infectious and parasitic diseases, Bacteria, Anaerobic, 03 medical and health sciences, Bacteria identification, Lactobacillus, Bacteroides, MALDI-TOF MS, lcsh:RC109-216, Anaerobes, Clostridium, biology, biology.organism_classification, Parabacteroides, Peptostreptococcus, 030104 developmental biology, Infectious Diseases, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Anaerobic bacteria, Research Article |
| الوصف: | Background Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been rapidly developed and widely used as an analytical technique in clinical laboratories with high accuracy in microorganism identification. Objective To validate the efficacy of MALDI-TOF MS in identification of clinical pathogenic anaerobes. Methods Twenty-eight studies covering 6685 strains of anaerobic bacteria were included in this meta-analysis. Fixed-effects models based on the P-value and the I-squared were used for meta-analysis to consider the possibility of heterogeneity between studies. Statistical analyses were performed by using STATA 12.0. Results The identification accuracy of MALDI-TOF MS was 84% for species (I2 = 98.0%, P 2 = 96.6%, P Bacteroides was the highest at 96% with a 95% CI of 95–97%, followed by Lactobacillus spp., Parabacteroides spp., Clostridium spp., Propionibacterium spp., Prevotella spp., Veillonella spp. and Peptostreptococcus spp., and their correct identification rates were all above 90%, while the accuracy of rare anaerobic bacteria was relatively low. Meanwhile, the overall capabilities of two MALDI-TOF MS systems were different. The identification accuracy rate was 90% for VITEK MS vs. 86% for MALDI biotyper system. Conclusions Our research showed that MALDI-TOF-MS was satisfactory in genus identification of clinical pathogenic anaerobic bacteria. However, this method still suffers from different drawbacks in precise identification of rare anaerobe and species levels of common anaerobic bacteria. |
| اللغة: | English |
| تدمد: | 1471-2334 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::685787304e90eb6fbc7359f76130d2bd http://link.springer.com/article/10.1186/s12879-019-4584-0 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....685787304e90eb6fbc7359f76130d2bd |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14712334 |
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