Mutations Affecting HVO_1357 or HVO_2248 Cause Hypermotility in Haloferax volcanii, Suggesting Roles in Motility Regulation
| العنوان: | Mutations Affecting HVO_1357 or HVO_2248 Cause Hypermotility in Haloferax volcanii, Suggesting Roles in Motility Regulation |
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| المؤلفون: | Mechthild Pohlschroder, Michiyah Collins, Aime Bienfait Igiraneza, Michael L. Dyall-Smith, Heather Schiller, Daniel P. Beiting, Friedhelm Pfeiffer, Simisola Afolayan, Elise Krespan |
| المصدر: | Genes, Vol 12, Iss 58, p 58 (2021) Genes Volume 12 Issue 1 |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Transposable element, transposon mutagenesis, Histidine Kinase, lcsh:QH426-470, archaea, Archaeal Proteins, hypermotility selection, 030106 microbiology, two-component regulatory system, Haloferax volcanii, Biology, swimming motility, Article, archaella, 03 medical and health sciences, Genome, Archaeal, Genetics, chemotaxis, Haloferax, Gene, Genetics (clinical), extremophiles, Whole Genome Sequencing, Histidine kinase, Chromosome Mapping, Computational Biology, biology.organism_classification, Two-component regulatory system, Response regulator, Mutagenesis, Insertional, lcsh:Genetics, 030104 developmental biology, Mutation, DNA Transposable Elements, Transposon mutagenesis |
| الوصف: | Motility regulation plays a key role in prokaryotic responses to environmental stimuli. Here, we used a motility screen and selection to isolate hypermotile Haloferax volcanii mutants from a transposon insertion library. Whole genome sequencing revealed that hypermotile mutants were predominantly affected in two genes that encode HVO_1357 and HVO_2248. Alterations of these genes comprised not only transposon insertions but also secondary genome alterations. HVO_1357 contains a domain that was previously identified in the regulation of bacteriorhodopsin transcription, as well as other domains frequently found in two-component regulatory systems. The genes adjacent to hvo_1357 encode a sensor box histidine kinase and a response regulator, key players of a two-component regulatory system. None of the homologues of HVO_2248 have been characterized, nor does it contain any of the assigned InterPro domains. However, in a significant number of Haloferax species, the adjacent gene codes for a chemotaxis receptor/transducer. Our results provide a foundation for characterizing the root causes underlying Hfx. volcanii hypermotility. |
| وصف الملف: | application/pdf; application/zip |
| اللغة: | English |
| تدمد: | 2073-4425 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::68b28fcdea3d149cfa3fb9e266490eef https://www.mdpi.com/2073-4425/12/1/58 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....68b28fcdea3d149cfa3fb9e266490eef |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20734425 |
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