Comparison of Reverse-Transcription Polymerase Chain Reaction Cycle Threshold Values From Respiratory Specimens in Symptomatic and Asymptomatic Children With Severe Acute Respiratory Syndrome Coronavirus 2 Infection
| العنوان: | Comparison of Reverse-Transcription Polymerase Chain Reaction Cycle Threshold Values From Respiratory Specimens in Symptomatic and Asymptomatic Children With Severe Acute Respiratory Syndrome Coronavirus 2 Infection |
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| المؤلفون: | Christopher R. Cannavino, Nanda Ramchandar, Teresa Mueller, Michelle K Vanderpool, Leidy Tovar Padua, John Strutner, Shruti Dubey, Alice Pong, Wei Wang, Mary Gamboa, Denise M. Malicki |
| المصدر: | Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Microbiology (medical), medicine.medical_specialty, Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), medicine.disease_cause, 01 natural sciences, Asymptomatic, cycle threshold, 03 medical and health sciences, 0302 clinical medicine, children, Internal medicine, Major Article, Medicine, Humans, 030212 general & internal medicine, 0101 mathematics, Respiratory system, Child, Coronavirus, Retrospective Studies, business.industry, Transmission (medicine), Reverse Transcriptase Polymerase Chain Reaction, SARS-CoV-2, 010102 general mathematics, COVID-19, Confidence interval, Reverse transcription polymerase chain reaction, Infectious Diseases, Cross-Sectional Studies, AcademicSubjects/MED00290, PCR, medicine.symptom, business, Viral load |
| الوصف: | Background Understanding viral kinetics of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is important to assess risk of transmission, manage treatment, and determine the need for isolation and protective equipment. The impact of viral load in asymptomatic infected children is important to understand transmission potential. We sought to determine whether children deemed to be asymptomatic had a difference in the polymerase chain reaction (PCR) cycle threshold (Ct) value of respiratory samples from symptomatic children with SARS-CoV-2 infection. Methods This was a retrospective cross-sectional study to compare PCR Ct values of children who tested positive for SARS-CoV-2 by respiratory samples collected over a 4-month period at a large tertiary care children’s hospital. Results We analyzed 728 children who tested positive for SARS-CoV-2 by reverse-transcription PCR (RT-PCR) from a respiratory sample over a 4-month period and for whom data were available in the electronic medical record. Overall, 71.2% of infected children were symptomatic. The mean Ct value for symptomatic patients (Ct mean, 19.9 [standard deviation, 6.3]) was significantly lower than for asymptomatic patients (Ct mean, 23.5 [standard deviation, 6.9]) (P < .001; 95% confidence interval, 2.6–4.6). The mean PCR Ct value was lowest in children Of 728 children who tested positive for severe acute respiratory syndrome coronavirus 2 by reverse-transcription polymerase chain reaction, mean cycle threshold (Ct) value was lower in symptomatic children. Children |
| تدمد: | 1537-6591 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6951d51842bf883dcb8759227a2696e3 https://pubmed.ncbi.nlm.nih.gov/34060629 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....6951d51842bf883dcb8759227a2696e3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15376591 |
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