Surveillance for Antibiotic-Resistant E. coli in the Salish Sea Ecosystem
| العنوان: | Surveillance for Antibiotic-Resistant E. coli in the Salish Sea Ecosystem |
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| المؤلفون: | James West, Jeffery Lahti, Michelle Wainstein, Stephanie A. Norman, Ryan Ruiz, Peter M. Rabinowitz, Marilyn C. Roberts, Scott J. Weissman, Marisa A D’Angeli, Dyanna M. Lambourn, Alexandria Vingino |
| المصدر: | Antibiotics Volume 10 Issue 10 Antibiotics, Vol 10, Iss 1201, p 1201 (2021) |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Microbiology (medical), antibiotic resistance, biology, English sole, Zoology, Virulence, Phocoena, RM1-950, biology.organism_classification, E. coli, Biochemistry, Microbiology, Phoca, river otters, Infectious Diseases, Antibiotic resistance, Lontra, Multilocus sequence typing, Pharmacology (medical), Therapeutics. Pharmacology, Typing, General Pharmacology, Toxicology and Pharmaceutics, marine mammals, environment |
| الوصف: | E. coli was isolated from the Salish Sea (Puget Sound) ecosystem, including samples of marine and fresh water, and wildlife dependent on this environment. E. coli isolates were assessed for phenotypic and genotypic resistance to antibiotics. A total of 305 E. coli isolates was characterized from samples collected from: marine water obtained in four quadrants of the Salish Sea select locations near beaches fresh water from streams near marine beaches and fecal samples from harbor porpoises (Phocoena phocoena), harbor seals (Phoca vitulina), river otters (Lontra canadensis), and English sole (Parophrys vetulus). Isolates were evaluated using antimicrobial susceptibility typing, whole-genome sequencing, fumC, and multilocus sequence typing. Resistance and virulence genes were identified from sequence data. Of the 305 isolates from Salish Sea samples, 20 (6.6%) of the E. coli were intermediate, and 31 (10.2%) were resistant to ≥1 class of antibiotics, with 26.9% of nonsusceptible (resistant and intermediate resistant) E. coli isolates from marine mammals and 70% from river otters. The proportion of nonsusceptible isolates from animals was significantly higher than samples taken from marine water (p < 0.0001). A total of 196 unique STs was identified including 37 extraintestinal pathogenic E. coli (ExPEC)-associated STs [ST10, ST38, ST58, ST69, ST73, ST117, ST131, and ST405]. The study suggests that animals may be potential sentinels for antibiotic-resistant and ExPEC E. coli in the Salish Sea ecosystem. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2079-6382 |
| DOI: | 10.3390/antibiotics10101201 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6958d6d70431013df29e4cb93b7f3b4e |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....6958d6d70431013df29e4cb93b7f3b4e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20796382 |
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| DOI: | 10.3390/antibiotics10101201 |